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中国农学通报

中国农学通报 ›› 2012, Vol. 28 ›› Issue (6): 131-134.

所属专题： 资源与环境； 小麦

• 农学 农业基础科学 • 上一篇    下一篇

ITS序列在苦荞麦种质资源鉴定中的应用

邵美红 孙加炎 林兵 秦路平   

  • 收稿日期:2011-08-23 修回日期:2011-10-31 出版日期:2012-02-25 发布日期:2012-02-25
  • 基金资助:

    浙江省种子工程项目

Application Research of ITS Sequences in Fagopyrum tataricum Identification

  • Received:2011-08-23 Revised:2011-10-31 Online:2012-02-25 Published:2012-02-25

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摘要：

为了更精确有效地鉴定苦荞麦种质资源，根据ITS序列通用引物，以苦荞麦基因组DNA为模板通过PCR方法扩增出ITS(internal translation spacer)序列，在克隆测序的基础上对荞麦属5个栽培苦荞麦中进行分子鉴定与亲缘关系分析。结果表明：（1）5个供试材料ITS序列的变异小，序列长度在584~590 bp之间，G+C含量高，为67.5%~68.5%。（2）通过聚类方法分析发现‘建德苦荞麦’和‘红花苦荞麦’聚为一支，‘西荞2号’和‘西荞3号’聚为一支。

关键词: 植物激素, 植物激素, 预处理技术, 分析检测

Abstract:

In order to identify germplasm resources of Fagopyrum tataricum, in this study the ITS general primers of sequence was used, F. tataricum genomic DNA as a template was amplified by PCR out of ITS sequences (internal translation spacer), the cloned sequence was based on 5 cultivars of F. Tataricum for molecular identification and molecular analysis of genetic relationship. The results showed that: (1) five materials were a small variation of ITS sequences, the length of sequences between 584-590 bp, G+C content was high up to 67.5%-68.5%. (2) Using different clustering analysis showed that ‘Jiande’ F. Tataricum and ‘Honghua’ F. Tataricum, ‘Xi 2’ F. Tataricum and ‘Xi 3’ F. Tataricumt had a closed genetic relationship.