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中国农学通报

中国农学通报 ›› 2012, Vol. 28 ›› Issue (6): 35-38.

所属专题： 生物技术； 水稻； 园艺

• 农学 农业基础科学 • 上一篇    下一篇

转甜菜碱醛脱氢酶基因宁夏粳稻幼苗生理研究

周晓燕 甘晓燕 石磊 宋玉霞   

  • 收稿日期:2011-09-21 修回日期:2011-11-22 出版日期:2012-02-25 发布日期:2012-02-25

Study on the Physiology of Transgenic Japonica Rice in Ningxia Seedlings with Gene of Betaine Aldehyde

  • Received:2011-09-21 Revised:2011-11-22 Online:2012-02-25 Published:2012-02-25

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摘要：

为了提高植物耐盐性，充分利用盐碱地，通过采用农杆菌介导法将超旱生耐盐植物梭梭(Haloxylon ammodendron)的甜菜碱醛脱氢酶基因（HaBADH）转入宁夏粳稻，对其阳性植株幼苗进行耐盐性方面的相关生理研究。结果表明，在高浓度NaCl（5.0，7.0 g/L）胁迫下，与对照相比，转HaBADH基因粳稻幼苗的超氧化物歧化酶(SOD)、过氧化物酶(POD)、过氧化氢酶(CAT)的活性显著提高；丙二醛(MDA)的含量显著降低。说明转入HaBADH基因提高了粳稻对盐胁迫的耐受性。

关键词: 银杏, 银杏, FLS 基因, FLS 基因启动子, 生物信息学

Abstract:

In order to improve plant salt tolerance, full use of saline-alkali soil, through the use of Agrobacterium tumefaciens ultra xeric salt-tolerant plants Haloxylon (Haloxylon ammodendron) of betaine aldehyde dehydrogenase gene (HaBADH) into Japonica rice of Ningxia, its positive seedings to salt tolerance in plants related to physiological research. The results showed that high concentrations of NaCl (5.0, 7.0 g/L) stress, compared with the control, transfer HaBADH gene rice seedings superoxide dismutase (SOD), peroxidase (POD), catalase (CAT) activity was significantly increased; malondialdehyde (MDA) levels were significantly reduced. The results showed that, transfer HaBADH gene could increase Japonica rice of Ningxia tolerance to salt stress.

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