欢迎访问《中国农学通报》，
中国农学通报

中国农学通报 ›› 2012, Vol. 28 ›› Issue (18): 266-270.doi: 10.11924/j.issn.1000-6850.2011-3587

所属专题： 生物技术

• 食品 营养 检测 安全 • 上一篇    下一篇

薏米蛋白提取及其SDS-PAGE电泳分析

尚宏丽 孟鑫 张挺   

  • 收稿日期:2011-12-01 修回日期:2012-02-03 出版日期:2012-06-25 发布日期:2012-06-25

Extraction and SDS-PAGE Analysis of Proteins from Job’s Tears Seed

  • Received:2011-12-01 Revised:2012-02-03 Online:2012-06-25 Published:2012-06-25

PDF (PC)

960

摘要：

为了提高薏米蛋白的提取得率，确定提取pH、料液比、提取温度、提取时间对蛋白提取率的影响，进一步得到薏米蛋白SDS-PAGE电泳图谱。采用碱法提取薏米蛋白，通过响应面回归分析，得到碱法提取薏米蛋白的优化工艺条件并且进行SDS-PAGE凝胶电泳分析薏米蛋白电泳图谱。结果表明，碱法提取薏米蛋白的优化工艺条件为料液比为1:11.8，提取时间4.93 h，提取温度为35℃，提取pH 10.47。在最优条件下，薏米蛋白提取率达到43.94%。薏米蛋白SDS-PAGE凝胶电泳分析，薏米蛋白电泳图谱共分离出2条蛋白亚基条带，较大薏米蛋白亚基分子量143 kD，较小薏米蛋白亚基分子量为85 kD。通过响应面碱法提取薏米蛋白，提取率明显提高，采用SDS-PAGE法对薏米蛋白进行分析，为构建薏米蛋白质指纹图谱提供理论依据。

关键词: 防效, 防效

Abstract:

In order to increase the protein extraction rate from Job’s Tears seed, determine the pH, solid-liquid ratio, treatment temperature, time on the extraction rate of protein effects, further barley protein SDS-PAGE electrophoresis. Based on single-factor test, the optimum extraction conditions were confirmed by response surface method, and made SDS-PAGE analysis of Job’s Tears seed electrophoresis. The results showed that, the optimum extraction conditions were confirmed by response surface method as follows: solid to liquid ratio 1:11.8, pH 10.47, treatment temperature 35℃, and time 4.93 h. Under these conditions, the extraction rate was 43.94%. The SDS-PAGE analysis showed that, the proteins from Job’s Tears seed contained approximately 2 subunits, larger barley protein subunit molecular weight was 143 kD, little barley protein subunit molecular weight was 85 kD. Through the response surface alkali extraction from Job’s Tears seed, extraction rate increased significantly, the SDS-PAGE method was adopted to analyze, to construct the protein fingerprint and provide a theoretical basis.