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中国农学通报

中国农学通报 ›› 2012, Vol. 28 ›› Issue (27): 167-171.

所属专题： 生物技术

• 生物技术科学 • 上一篇    下一篇

香蕉枯萎病菌果胶裂解酶基因的克隆与序列分析

董章勇 王振中   

  • 收稿日期:2012-02-06 修回日期:2012-03-13 出版日期:2012-09-25 发布日期:2012-09-25
  • 基金资助:

    国家自然科学基金;公益性行业（农业）科研专项

Cloning and Comparative Analysis of Pectate lyase Gene from Fusarium oxyporum f. sp. cubense

  • Received:2012-02-06 Revised:2012-03-13 Online:2012-09-25 Published:2012-09-25

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摘要：

为了解香蕉枯萎病菌(Fusarium oxysporum f.sp. cubense)4号生理小种(FOC4)PL基因序列特征，根据同源物种PL相关序列设计引物，利用PCR和RT-PCR技术，克隆了FOC4序列基因，命名为pl2-FOC4。PL基因DNA序列由3个内含子和4个外显子组成。其cDNA编码区包含了831 bp的片段，编码276个氨基酸。预测编码蛋白有信号肽，其分子质量和等电点分为30271.9 Da和5.06，该蛋白为稳定存在的蛋白。PL2-FOC4具有5个N-糖基化位点，4个蛋白激酶C磷酸化位点，5个酪蛋白激酶Ⅱ磷酸化位点，3个豆蔻酸位点。该基因编码的蛋白具有一定保守性，进化上与镰刀菌亲缘关系最近。

关键词: 相关性, 相关性

Abstract:

To understand the Fusarium oxysporum f.sp. cubense race 4 (FOC4) Pectate lyase (PL) gene sequence features, a pair of primer that according to the homologous gene sequence documented in GenBank was designed to amplify the PL gene. Using PCR and RT-PCR, the genome and open reading frame (ORF) from FOC4 was successfully amplified and named it pl2-FOC4. The gene included 3 introns and 4 exons. The cDNA encoding region was composed by 831 bases and encoded 276-amino acid polypeptide with 30271.9 Da of calculated molecular weight and 5.06 of pI. PL2-FOC4 got five N-glycosylation site, four Protein kinase C phosphorylation site, five Casein kinase Ⅱ phosphorylation site and three N-myristoylation site. The protein with evolutionary conservative and got the closest relatives with Fusarium oxyporum.