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中国农学通报

中国农学通报 ›› 2012, Vol. 28 ›› Issue (33): 135-138.

所属专题： 水稻

• 农学 农业基础科学 • 上一篇    下一篇

用SSR标记鉴定水稻突变体杂交F1的研究

查仁明 王启元 罗洪发 王文挺 龙天喜 江燕   

  • 收稿日期:2012-02-23 修回日期:2012-05-02 出版日期:2012-11-25 发布日期:2012-11-25
  • 基金资助:

    贵州省科学技术基金;贵州大学人才基金

Identification of F1 from rice (Oryza sativa L.) mutants by SSR markers

  • Received:2012-02-23 Revised:2012-05-02 Online:2012-11-25 Published:2012-11-25

PDF (PC)

861

摘要：

DNA标记技术可从基因组水平上鉴定F1。用SSR标记鉴定水稻突变体杂交F1，为F2群体构建和突变体基因定位提供依据。以经EMS诱导获得的R30白化突变和‘泸恢17’窄叶突变分别作为亲本，组配2个杂交组合，用3个SSR标记对其单株进行鉴定。RM6105检测了R30白化突变ב泸恢17’组合4个单株（1~4号），其中4号单株表现父母本互补的带型，为真实F1，另外3个的标记型与母本相同，为母本自交结实，RM6965和RM5349的鉴定结果与RM6105一致；该3个标记从R30ב泸恢17’窄叶突变组合14个F1单株中（5~18号）检测到3个真实F1（9、12和15号）；RM6965、RM5349和RM6105均可对R30与‘泸恢17’组合F1进行鉴定，且结果一致；分子标记对F1进行鉴定，可用于杂交育种。

关键词: 果实品质, 果实品质

Abstract:

F1 could be identified by DNA marker technology in genome. To provide the basis for F2 group construction and gene location of mutants, F1 hybridized with rice mutants were identified by SSR markers. Mutants of albefaction from R30 and of narrow leaf from ‘Luhui 17’ induced by EMS were used as parents respectively in two cross-combinations. The F1 plants from the two combinations were respectively identified by three SSR markers. The four F1 plants (code 1-4) from mutation of albefaction from R30בLuhui 17’ were identified by RM6105. The code 4 showed complementary marker type of both parents. This indicated it was real F1. The marker types of other three were the same as its female parent. They were self-pollinated for female parent. The results of identification by RM6965 and RM5349 were consistent with that by RM6105. Three (code 9, 12 and 15) from the fourteen F1 (code 5-18) from R30×Mutation of narrow leaf from ‘Luhui 17’ were identified to be real F1. The F1 crossed with R30 and ‘Luhui 17’ could be identified respectively by the three markers of RM6965, RM5349 and RM6105 and the results were same. The F1 identification with molecular marker could be applied in cross-breeding.