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中国农学通报

中国农学通报 ›› 2012, Vol. 28 ›› Issue (29): 71-74.

所属专题： 生物技术

• 畜牧 动物医学 蚕 蜂 • 上一篇    下一篇

抗猫杯状病毒单克隆抗体的制备及生物学特性鉴定

艾纯旭 蓝田丰 孟令伟 衣帅 樊小烨 朱拓 谢珊珊 高巍 袁宝 陈建 胡进平 任文陟 刘殿峰   

  • 收稿日期:2012-04-20 修回日期:2012-06-07 出版日期:2012-10-15 发布日期:2012-10-15
  • 基金资助:

    吉林省实验动物质量检测中心平台建设

Preparation of Monoclonal Antibodies against Feline Calicivirus and Their Identification of Biological Characters

  • Received:2012-04-20 Revised:2012-06-07 Online:2012-10-15 Published:2012-10-15

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摘要：

为了制备抗猫杯状病毒的单克隆抗体，并对其基本生物学特性进行鉴定。分别采用饱和硫酸铵方法、差速离心、氯化铯密度梯度离心方法对猫杯状病毒(FCV)进行纯化，将纯化的猫杯状病毒作为抗原对BALB/c小鼠进行免疫，通过细胞融合和杂交瘤筛选技术建立抗猫杯状病毒单克隆抗体的杂交瘤系，鉴定单克隆抗体的亚型。结果表明：本实验获得了2株稳定分泌特异性抗猫杯状病毒单克隆抗体的杂交瘤细胞，分别命名为D8、E5，其亚型均为IgM。获得的单克隆抗体与犬细小病毒(CPV)、猫细小病毒(FPV)、犬瘟热病毒(CDV)均无交叉反应。成功制备了抗猫杯状病毒单克隆抗体，为建立相关诊断方法奠定了基础。

关键词: 相关性, 相关性

Abstract:

To prepare Monoclonal Antibodies against Feline Calicivirus and identify their basic biological characteristics. By saturated ammonium sulfate method, differential centrifugation and chlorinated cesium density gradient centrifugation to Purify Feline Calicivirus (FCV), BALB/c mice were immunized by the purified Feline Calicivirus, the hybridoma cells secreting anti-feline Calicivirus monoclonal antibody were established by cell fusion and hybridoma screening technique. The results showed that: two Hybridoma Cells which could stably secret Monoclonal Antibody against FCV were obtained and named D8 and E5, the isotype of both two monoclonal antibodies was IgM, the two monoclonal antibodies had no cross-reaction with canine parvovirus (CPV), Feline Panleukopenia Virus (FPV) and Canine Distemper Virus (CDV). Successfully prepared the Monoclonal Antibodies against Feline Calicivirus, which laid the foundation for the establishment of relevant diagnostic method.