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中国农学通报 ›› 2014, Vol. 30 ›› Issue (27): 114-118.doi: 10.11924/j.issn.1000-6850.2014-0860

所属专题: 油料作物

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福建省野生大豆耐盐种质的筛选

张玉梅,胡润芳,林国强   

  1. 福建省农业科学院作物研究所,福建省农业科学院作物研究所,福建省农业科学院作物研究所
  • 收稿日期:2014-03-27 修回日期:2014-03-27 接受日期:2014-05-30 出版日期:2014-10-15 发布日期:2014-10-15
  • 通讯作者: 林国强
  • 基金资助:
    福建省农科院导师制青年基金项目“福建省野生大豆耐盐种质的筛选及蛋白质组学研究”(2012DQB-27);福建省农科院科技创新团队建设项目“粮经作物种质创新及其利用”(CXTD-1-1301);福建省科技厅区域重大项目“特色蔬菜(菜用大豆、玉米)节本增效安全栽培及冷链保质保鲜技术研究与应用”(2013N3013);福建省科技计划重大专项“水土流失初步治理区植被修复与特色种植业产业提升及示范园建设”(2012NZ0002-1)

Screening of Glycine soja Germplasm from Fujian Province for Salt Tolerance

  • Received:2014-03-27 Revised:2014-03-27 Accepted:2014-05-30 Online:2014-10-15 Published:2014-10-15

摘要: 盐害是一种重要的非生物胁迫之一,限制植物的生长并降低作物的产量和品质,野生大豆是栽培大豆的近缘野生种,含有诸多优异的基因。为评价野生大豆的耐盐性,试验采用漂浮育苗方法,以NaCl致死浓度为评价指标,对244 份野生大豆苗期的耐盐性进行鉴定。结果表明野生大豆种质致死浓度最低为200 mmol/L,最高为300 mmol/L,并从中筛选出致死浓度为290 mmol/L 耐盐种质14 份,占供试材料的6%,致死浓度为300 mmol/L 的耐盐种质2 份,占供试材料的1%;致死浓度为200 mmol/L 的盐敏感种质15 份,占供试材料的6%;致死浓度为270 mmol/L的材料最多,共计36 份,占供试材料的15%,其次是致死浓度为220 mmol/L 的材料32 份,致死浓度为280 mmol/L 的材料29 份。综上表明野生大豆种质资源耐盐性差异较大,含有丰富的遗传变异,有望筛选出优异的耐盐种质,用于耐盐QTL的定位和耐盐机理的研究。

关键词: 染色体, 染色体

Abstract: Salt stress is one of the most important abiotic stresses that limit the growth of plants and reduce crop yield and quality. Wild soybeans are wild relatives of cultivated soybean and possess many excellent genes. To evaluate the salt tolerance of wild soybeans, using float seedling technology and taking NaCl lethal concentration as evaluation index, 244 Glycine soja germplasms from Fujian Province were evaluated for salt tolerance at seedling stage. The results showed that the lowest and highest of NaCl lethal concentration were 200 mmol/L and 300 mmol/L, respectively. Among these germplasms, 14 with salt tolerance were screened out with NaCl lethal concentration of 290 mmol/L, representing 6% of the total test materials, only 2 with 300 mmol/L lethal concentration, accounting for 1%; 15 germplasms with salt sensitivity had 200 mmol/L lethal concentration, accounting for 6% of the total test materials. 36 test materials had lethal concentration of 270 mmol/L, accounting for 15%, and 32 test materials had lethal concentration of 220 mmol/L and 29 had lethal concentration of 280 mmol/L. In summary, wild soybean were significantly different in salt tolerance and rich in genetic variation, and could be expected to screen out excellent salt-tolerant germplasm for the study of salt tolerant QTL and mechanism.