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中国农学通报 ›› 2015, Vol. 31 ›› Issue (10): 138-141.doi: 10.11924/j.issn.1000-6850.casb14100019

所属专题: 生物技术

• 林学 园艺 园林 • 上一篇    下一篇

百合鳞茎形成的生理生化研究

朱志国,季晓莲   

  1. (芜湖职业技术学院园林园艺学院,安徽芜湖 241003)
  • 收稿日期:2014-10-12 修回日期:2015-01-27 接受日期:2015-02-10 出版日期:2015-05-05 发布日期:2015-05-05
  • 通讯作者: 朱志国
  • 基金资助:
    安徽高校省级自然科学研究项目基金“百合优质种球繁殖技术研究”(KJ2012B219);安徽省高校青年教师资助计划项目“蝴蝶兰组织培养快速繁殖技术研究”(2008jq1182)。

Physiological and Biochemical Research on Lily Bulb Formation

Zhu Zhiguo, Ji Xiaolian   

  1. (Department of Landscape Architecture and Horticulture, Wuhu Institute of Technology, Wuhu Anhui 241003)
  • Received:2014-10-12 Revised:2015-01-27 Accepted:2015-02-10 Online:2015-05-05 Published:2015-05-05

摘要: 为研究建立高效稳定的百合鳞茎形成体系,以自繁的东方百合(Lilium Oriental Hybrid)品种‘索蚌’为材料,研究SOD、POD、CAT 3种抗氧化酶的活性变化以及可溶性蛋白和可溶性糖含量变化对百合鳞茎发生过程中的生理生化机制。结果表明,在百合鳞茎发生发育过程中,SOD与POD活性升高,而CAT酶活性的变化与SOD、POD活性变化趋势正好相反;可溶性蛋白累积和可溶性糖变化与百合鳞茎形成密切相关。因此,在百合鳞茎形成过程中,SOD、POD、CAT酶活性变化与鳞茎诱导及其发育密切相关,其中SOD、POD酶在百合鳞茎形成的过程中起着主导作用;可溶性糖和可溶性蛋白质累积变化为百合鳞茎形成提供物质和能量基础。

关键词: 澳洲坚果, 澳洲坚果, 火焰原子吸收分光光度法, 微量元素

Abstract: In order to research and establish highly stable formation system, SOD activity, POD activity, CAT activity, soluble protein and soluble sugar contents were studied to investigate their effects on physiological and biochemical mechanism of oriental lily ‘Sorbonne’. The results indicated that during the growth and development of lily bulb, SOD and POD activities increased, CAT activity decreased, and soluble protein accumulation and soluble sugar contents changes were closely related to the Lily bulb formation. Therefore, the changes of SOD, POD and CAT activities were closely related to the bulb induction and formation with SOD and POD activities playing leading roles in the process of lily bulb formation; soluble sugar and soluble protein accumulation provided material and energy basis for lily bulb development.