欢迎访问《中国农学通报》，
中国农学通报

中国农学通报 ›› 2015, Vol. 31 ›› Issue (22): 184-190.doi: 10.11924/j.issn.1000-6850.casb15030047

所属专题： 烟草种植与生产； 农业生态

• 植物保护 农药 • 上一篇    下一篇

转Lchi1烟草T2代对梨腐烂病菌的抗性研究

李伟阳1,曾 斌1,蒋 萍1,李 疆1,李秀根2,王苏珂2   

  1. (1新疆农业大学林学与园艺学院/新疆农业大学果树学新疆特色果树研究中心,乌鲁木齐 830052；2中国农业科学院郑州果树研究所,郑州 450009）
  • 收稿日期:2015-03-07 修回日期:2015-05-18 接受日期:2015-05-25 出版日期:2015-08-20 发布日期:2015-08-20
  • 通讯作者: 曾斌
  • 基金资助:
    国家高技术研究发展计划（863计划）子课题“梨分子育种与品种创新”(2011AA10020604)；新疆维吾尔自治区果树学重点学科(201007)。

Resistance Research of Transgenic Tobacco T2 Generation with Lchi1 Against Valsa ceratosperma

Li Weiyang1, Zeng Bin1, Jiang Ping1, Li Jiang1, Li Xiugen2, Wang Suke2   

  1. (1Collage of Forestry and Horticultural, Xinjiang Agricultural University/ The Characteristics Fruit Tree Research Center, Xinjiang Agricultural University, Urumqi 830052; 2Zhengzhou Fruit Research Institute, Chinese Academy of Agriculture Sciences, Zhengzhou 450009)
  • Received:2015-03-07 Revised:2015-05-18 Accepted:2015-05-25 Online:2015-08-20 Published:2015-08-20

PDF (PC)

501

摘要： 获得转化梨几丁质酶基因Lchi1的烟草，检测组成型表达Lchi1基因烟草是否能提高对梨腐烂病菌(Valsa ceratosperma)的抗性，从而初步确定Lchi1基因的功能，为进一步开展梨抗腐烂病分子育种工作提供参考。构建梨几丁质酶基因Lchi1的植物表达载体，通过农杆菌介导的叶盘法转化烟草，进而对转化的烟草T2代进行PCR及RT-PCR检测。利用离体叶片接种法，更直观地验证转基因烟草对梨腐烂病菌的抗性。Lchi1基因已经整合到烟草基因组中，并在烟草T2代中有效表达，在接种腐烂病菌后转基因烟草较未转基因烟草的抗腐烂病菌能力明显增强。梨几丁质酶基因Lchi1与腐烂病抗性相关联，可作为今后梨的抗腐烂病分子育种研究重要的基因来源。

关键词: 大麻, 大麻, ISSR, 反应体系, 优化

Abstract: The study aims to obtain the transgenic tobacco containing Lchi1 which is the chitinase gene, and detect whether the transgenic tobacco with constitutive Lchi1 gene expression can improve resistance to Valsa ceratosperma, thus to preliminarily determine the function of Lchi1 gene and provide a reference for the further research on molecular breeding about pear Valsa canker. Plant expression vector with gene Lchi1 was constructed and transformed into tobacco by leaf disc mediated with Agrobacterium tumefaciens, and transgenic tobacco T2 generation was detected by PCR and RT-PCR. The resistance of transgenic tobacco against Valsa ceratosperma was verified more intuitively by isolated leaf inoculation method. Lchi1 was integrated into tobacco genome, and it was expressed effectively in the transgenic tobacco T2 generation. After inoculating Valsa ceratosperma, the resistance of transformant against Valsa ceratosperma was obviously more enhanced compared with non transgenic tobacco. In conclusion, Lchi1, which is the pear chitinase gene, is associated with the resistance of pear Valsa canker, and it will be used as an important genetic source in the research of molecular resistance breeding.