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中国农学通报 ›› 2015, Vol. 31 ›› Issue (32): 92-98.doi: 10.11924/j.issn.1000-6850.casb15060053

所属专题: 油料作物

• 植物保护 农药 • 上一篇    下一篇

连作大豆苗期根际土壤根腐病病原真菌菌群结构分析

蔡柏岩1,2,王丽阳1,2,胡 维1,2,接伟光1,2,凌宏志1,2   

  1. (1黑龙江大学生命科学学院,哈尔滨 150080;2黑龙江省普通高等学校微生物重点实验室,哈尔滨 150080)
  • 收稿日期:2015-06-09 修回日期:2015-07-17 接受日期:2015-07-24 出版日期:2015-11-16 发布日期:2015-11-16
  • 通讯作者: 蔡柏岩
  • 基金资助:
    黑龙江省教育厅科学技术研究项目资助“连作大豆土壤AM真菌菌群结构分析及其与根腐病病原菌相互关系的研究”(12521394)。

Analysis of Community Structure of Root Rot Pathogenic Fungi in Seedling Stageof Soybean Continuous Cropping

Cai Baiyan1,2, Wang Liyang1,2, Hu Wei1,2, Jie Weiguang1,2, Ling Hongzhi1,2   

  1. (1College of Life Science, Heilongjiang University, Harbin 150080; 2Key Laboratory of Microbiology of General Institutes of Higher Education, Harbin 150080)
  • Received:2015-06-09 Revised:2015-07-17 Accepted:2015-07-24 Online:2015-11-16 Published:2015-11-16

摘要: 连作障碍是限制黑龙江地区大豆产质量提高的主要因素。本项研究的主要目的是探究连作大豆根腐病发病期病原真菌菌群结构,为生物防治大豆根腐病奠定基础。采用PCR-DGGE技术,分析不同连作年限苗期大豆土壤中的3个大豆品种根系以及根际土壤根腐病病原真菌菌群结构。试验结果表明:随着连作年限的延长,3个大豆品种苗期根系以及根际土壤根腐病病原真菌丰度值以及多样性指数随之增加;并且不同大豆品种间根腐病病原真菌菌群结构也存在差异;聚类分析结果验证了连作年限、大豆品种均对根腐病病原真菌菌群结构产生影响。

关键词: 秸秆, 秸秆, 近红外, 模型传递

Abstract: Continuous cropping obstacle is the main factor that restricts the improvement of soybean quality and yield in Heilongjiang region. The aim of this study was to investigate the community structure of root rot pathogenic fungi in the continuous cropping of soybean which could lay a foundation for bio-control of the soybean root rot. In this study, the community structure of root rot pathogen in the seedling stage of root and soil samples of three soybean varieties planted in different duration of continuous cropping soil was analyzed by PCR-DGGE technology. The result showed that along with the extension of continuous cropping duration, the index of abundance and diversity of the three soybean varieties of root rot pathogen was increased in the seedling stage of root and soil samples; furthermore, the community structure of root rot pathogen was different among the soybean varieties. The result of cluster analysis verified that both the duration of continuous cropping and soybean variety had an effect on the community structure of root rot pathogen.

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