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中国农学通报

中国农学通报 ›› 2016, Vol. 32 ›› Issue (4): 1-4.doi: 10.11924/j.issn.1000-6850.casb15070136

• 林学 园艺 园林 •    下一篇

60Co-γ射线辐照处理对杉木种子萌发及幼苗生长的影响

胡瑞阳1,吴 博1,纳 静1,段红静1,方禄明2,余小龙2,孙宇涵1,李 云1   

  1. (1北京林业大学林木育种国家工程实验室/林木花卉遗传育种教育部重点实验室/北京林业大学生物科学与技术学院,北京 100083；2福建省将乐国有林场,福建将乐 353300）
  • 收稿日期:2015-07-23 修回日期:2016-01-04 接受日期:2015-08-18 出版日期:2016-02-01 发布日期:2016-02-01
  • 通讯作者: 李云
  • 基金资助:
    国家“863”重点项目“落叶松、马尾松、杉木分子育种及品种创制”(2011AA100203)；国家林业局重点项目“杉木诱变育种技术”(2012-06)。

Effects of 60Co-γ Radiation on Seed Germination and Seedling Growth of Cunninghamia lanceolata

Hu Ruiyang1, Wu Bo1, Na Jing1, Duan Hongjing1, Fang Luming2, Yu Xiaolong2, Sun Yuhan1, Li Yun1   

  1. (1National Engineering Laboratory for Tree Breeding/Key Laboratory of Genetics and Breeding in Forest Trees and Ornamental Plants, Ministry of Education/College of Biological Sciences and Technology, Beijing Forestry University, Beijing 100083;2Jiangle National Forest Farm, Jiangle Fujian 353300)
  • Received:2015-07-23 Revised:2016-01-04 Accepted:2015-08-18 Online:2016-02-01 Published:2016-02-01

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摘要： 为了探讨60Co-γ辐射剂量对杉木种子萌发及幼苗生长的影响，本研究以杉木第3 代种子园收集的种子为供试材料，研究了不同剂量的γ射线辐照处理对杉木种子发芽率、发芽时间、出苗率以及诱变苗早期生长的影响。结果表明：20 Gy辐照明显促进发芽，随着剂量的增加发芽率逐渐降低，80 Gy是致死剂量；辐照剂量20~60 Gy之间时，随着剂量的增加发芽时间会逐渐延长，出苗率会逐渐降低；γ射线辐照处理获得的诱变苗株高、地径和分枝数同样受剂量效应的影响，随着辐照剂量的增加，表现在植株矮化，地径变细，分枝数先升高后降低，尤其是40 Gy的辐照剂量能够显著促进分枝。根据辐照剂量与发芽率、出苗率可以初步确定，γ射线辐射杉木种子的半致死剂量在40~60 Gy之间。

关键词: 冷浸田, 冷浸田, 氧化还原电位, 活性还原性物质, 石灰, 有机肥, 秸秆

Abstract: In order to investigate the effects of different doses of 60Co-γ radiation on seed germination and seedling growth of Cunninghamia lanceolata, the seeds collected from the third generation Cunninghamia lanceolata seed orchard were taken as materials to test the effects of different doses of gamma irradiation treatments on the seed germination rate, germination time, seedling emergence rate and the mutagenic seedling growth of Cunninghamia lanceolata. The results showed that: 20 Gy radiation obviously accelerated the germination but the germination rate gradually decreased with the increase of the dose put into application and 80 Gy was a lethal dose; with the dose increase, the germination time gradually extended while the seedling emergence rate gradually decreased when the irradiation dose was between 20 Gy and 60 Gy. Different gamma irradiation doses also had an effect on the height, ground diameter and branch number of the mutated plants, with the increase of irradiation dose, the plants tended to be dwarfed and the ground diameter decreased, while the number of branches first increased and then decreased; in particular, the 40 Gy irradiation dose could significantly accelerate branching. It can be preliminarily ascertained that, according to the irradiation doses, the germination rate and the seedling emergence rate, the half lethal dose of gamma-ray radiation on Cunninghamia lanceolata seeds is between 40 Gy and 60 Gy.

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