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中国农学通报 ›› 2017, Vol. 33 ›› Issue (15): 71-75.doi: 10.11924/j.issn.1000-6850.casb16110139

• 林学 园艺 园林 • 上一篇    下一篇

甜杨离体再生中玻璃化苗的发生与防治

赵鑫闻,梁德军   

  1. (辽宁省杨树研究所,辽宁盖州 115200)
  • 收稿日期:2016-11-28 修回日期:2017-05-05 接受日期:2017-04-19 出版日期:2017-05-26 发布日期:2017-05-26
  • 通讯作者: 赵鑫闻
  • 基金资助:
    国家重点研发课题“辽宁渤海地区欧美杨工业资源材高效培育技术研究”(2016YFD0600401);辽宁省科学事业公益研究基金项目“黑杨花粉管通道导入外源DNA及其细胞学机理研究”(2011005007)。

The Occurrence and Control of Vitrification Shoots of Populous suaveolens in Vitro Regenerative Culture

Zhao Xinwen, Liang Dejun   

  1. (Research Institute of Poplar of Liaoning Province, Gaizhou Liaoning 115200)
  • Received:2016-11-28 Revised:2017-05-05 Accepted:2017-04-19 Online:2017-05-26 Published:2017-05-26

摘要: 由于甜杨不定芽的诱导过程中发生了玻璃化现象,为了恢复和逆转甜杨玻璃化苗,本试验采用正交试验设计L9(34)对影响玻璃化现象发生的光照强度、琼脂浓度和蔗糖浓度等因子进行研究。结果表明,琼脂浓度对甜杨玻璃化苗恢复率的影响最大,达到极显著水平,蔗糖浓度和光照强度对甜杨玻璃化苗的恢复率影响不显著。当琼脂浓度为9 g/L,培养光照强度为3000 lx,培养基中蔗糖浓度为20 g/L时,甜杨玻璃化苗的恢复率最高,达到100%。由此可知,培养基中较高的琼脂浓度对甜杨玻璃化苗的防治与逆转有显著作用。

关键词: 油茶, 油茶, 施肥, 配方施肥, 营养诊断

Abstract: The vitrification of populous suaveolens was found during tissue culture. The influence factors on the reversion of vitrification were studied using orthogonal experimental design, include culture illumination, concentration of sucrose and agar. The results showed that concentration of sucrose have significant influence on the the reversion of vitrification, while the concentration of agar and the culture illumination have little. The rate of reversion can reach the highest 100% in the medium that added 9g/L sucrose, 20g/L agar, and with the culture illumination 3000lx. As a result, it is concluded that the medium adding higher concentration of sucrose would have great effect on the reversion of populous suaveolens.

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