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中国农学通报

中国农学通报 ›› 2018, Vol. 34 ›› Issue (9): 53-56.doi: 10.11924/j.issn.1000-6850.casb17060077

所属专题： 园艺

• 生物技术科学 • 上一篇    下一篇

甜菜SCoT核心引物的筛选

兴旺,乔晓晓,马龙彪,吴则东   

  1. 黑龙江大学农作物研究院;中国农业科学院甜菜研究所,黑龙江大学,黑龙江大学,黑龙江大学
  • 收稿日期:2017-06-14 修回日期:2017-07-17 接受日期:2017-07-21 出版日期:2018-03-21 发布日期:2018-03-21
  • 通讯作者: 吴则东
  • 基金资助:
    国家现代农业产业技术体系建设项目“东北区育种岗位专家”(CARS-210104)；农业部物种资源保护费项目“甜菜种质资源收集鉴定编目 繁种保存与利用”(1120162130135252026)；科技部、财政部国家科技基础条件平台项目“国家甜菜种质资源平台”(NICGR2017-17)。

Selection of SCoT Core Primer for Beet

  • Received:2017-06-14 Revised:2017-07-17 Accepted:2017-07-21 Online:2018-03-21 Published:2018-03-21

摘要： 【研究目的】为了筛选出适合甜菜品种遗传多样性分析的SCoT引物，为SCoT引物得以在甜菜中进行深入应用奠定基础，本研究甜菜以8个来自不同国家的甜菜品种为材料对80条SCoT引物进行扩增【方法】扩增方法采用SCoT-PCR最优反应体系包含2.0 μL的10×PCR buffer（含Mg2+）、10 ng的DNA、0.5 U的Taq DNA聚合酶、10 μmol/L的引物2 μL以及0.1 μL的dNTPs (2.5 mmol/L each)。【结果】结果从80条SCoT引物中筛选出20条多态性高的引物，这20条引物最多扩增出16条多态性条带，最少扩增出2条多态性条带，扩增总条带155条，其中多态性条带为134条，多态性条带的百分比为86.5%。【结论】通过对核心引物的有效性验证，表明筛选出的20个核心引物非常适合用于甜菜指纹图谱的构建。

关键词: 青刺果, 青刺果, 生物学特征, 化学成分, 低温萃取技术

Abstract: The aim is to select the SCoT primer that suitable for the genetic diversity analysis of beet varieties. In this study, eight beet varieties from different countries were used as materials, and 80 SCoT primers were amplified. The optimal reaction system of SCoT-PCR was used in this study, it included 2.0 μL of 10×PCR buffer (Mg2+), 10 ng of template DNA, 0.5 U ofTaq DNA, 2 μL of 10 μmol/L primers and 0.1 μL of dNTPs (2.5 mmol/L each). As a result, 20 primers with high level of polymorphism were selected from 80 primers. In the 20 primers, some one amplified 16 polymorphic bands at most, and some one amplified 2 polymorphic bands at least. The total bands were 155, including 134 polymorphic bands, with a polymorphism rate of 86.5%. Through the validation of the core primer, the 20 core primers selected are suitable for the fingerprint construction of beet.

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