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中国农学通报

中国农学通报 ›› 2006, Vol. 22 ›› Issue (4): 40-40.

所属专题： 生物技术； 油料作物

• 目次 • 上一篇    下一篇

农杆菌介导法转化大豆体细胞胚获得转基因植株

王晓春,,王 罡,王 萍,季 静,刘尚前   

  • 出版日期:2006-04-05 发布日期:2006-04-05

Genetic Transformation of somatic embryos viaAgrobacterium tumefaciens in Soybean

Wang Xiaochun,, Wang Gang, Wang Ping, Ji Jing, Liu Shangqian   

  • Online:2006-04-05 Published:2006-04-05

PDF (PC)

748

摘要： 以六个品种大豆体细胞胚为受体，以Bt基因为目的基因，应用根癌农杆菌介导法对大豆进行了遗传转化，经卡那霉素选择、诱导体细胞胚萌发及壮苗培养，获得了完整的抗性再生苗，经PCR、PCR-Southern和点杂交分析鉴定，初步证明外源基因Bt已整合到大豆的基因组中。

关键词: 磁场, 磁场, 水稻, 种子, NaCl胁迫溶液, 种子萌发

Abstract: Somatic embryos of 6 different soybean were transformed by Agrobacterium tumefaciens , The resistant plants to kanamycin were obtained. 5 positive plants were obtained among them by PCR , These positive plantlets were further analyzed by dot blotting and PCR-Southern blotting. These results demonstrated that the target gene had been transformed into the soybean genome.

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