基于ISSR标记的5个蓼蓝居群遗传多样性分析

doi:10.11924/j.issn.1000-6850.casb2020-0765

摘要/Abstract

摘要：

旨在分析5个不同居群蓼蓝的遗传多样性及亲缘关系,为蓼蓝种质资源的保护和开发利用提供分子依据。以5个不同居群的蓼蓝为实验材料,利用简单重复序列(ISSR)分子标记技术进行遗传多样性分析;使用Popgene 32软件对扩增结果进行分析;使用NTsys2.10e软件按非加权配对算数平均法(UPGMA)构建居群间遗传距离聚类图。结果表明,23条引物共扩增出155个位点,其中148个为多态性位点;Nei′s基因多样性指数(H)为0.3538,Shannon多样性指数(I)为0.5234,基因分化系数(Gst)为0.4096,基因流(Nm)为0.7206;以遗传距离0.234为阈值可将5个居群分为三类,其中日本居群和其他居群间显示出较大差异。本研究结果显示蓼蓝居群间遗传差异与地理距离呈现一定相关性,对蓼蓝遗传多样性及种质资源的保护利用提供了理论基础。

关键词: 蓼蓝, ISSR分子标记, 遗传多样性, 聚类分析

Abstract:

The research aims at analyzing genetic diversity and relationship of 5 Polygonum tinctorium Ait. populations, and providing molecular references to the germplasm resources protection, exploitation and utilization of P. tinctorium Ait.. 5 P. tinctorium Ait. populations were used as materials. The genetic diversity was studied by simple inter-sequence repeat (ISSR) makers. The software Popgene 32 was used to analyze the amplification results, and the NTsys2.10e was used to construct a cluster map according to the unweighted pair-group method with arithmetic means (UPGMA). The results showed that a total of 155 loci were amplified by 23 primers, of which 148 were polymorphic loci; the Nei's genetic diversity coefficient (H) was 0.3538, Shannon's Information index (I) was 0.5234, the coefficient of genetic differentiation (Gst) was 0.4096, and the gene flow (Nm) was 0.7206. Taking the genetic distance 0.234 as a threshold, the 5 populations were divided into three groups, among which the Japanese population was different from others. The results indicate that the genetic difference among the populations of P. tinctorium Ait. is related to the geographical distance. This study could provide a theoretical basis for the genetic diversity of P. tinctorium Ait. and the protection and utilization of germplasm resources.

Key words: Polygonum tinctorium Ait., ISSR molecular marker, genetic diversity, cluster analysis

中图分类号:

徐明, 汪琼, 李冬玲, 郑玉红, 徐增莱. 基于ISSR标记的5个蓼蓝居群遗传多样性分析[J]. 中国农学通报, 2021, 37(21): 134-139.

Xu Ming, Wang Qiong, Li Dongling, Zheng Yuhong, Xu Zenglai. 5 Polygonum tinctorium Ait. Populations: Genetic Diversity Analysis Based on ISSR Marker[J]. Chinese Agricultural Science Bulletin, 2021, 37(21): 134-139.

图/表 6

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序号	编号	采集地	样本量
1	SC	四川省凉山彝族自治州布拖县	5
2	SD	山东省日照市	5
3	JS	江苏省南通港闸区	5
4	GZ	贵州省黎平县	5
5	RB	日本德岛县（引种于本园）	5

序号	编号	采集地	样本量
1	SC	四川省凉山彝族自治州布拖县	5
2	SD	山东省日照市	5
3	JS	江苏省南通港闸区	5
4	GZ	贵州省黎平县	5
5	RB	日本德岛县（引种于本园）	5

引物名称	序列(5'-3')	总位点数	多态性位点数	多态性位点百分率/%
801	(AT)8T	4	4	100
803	(AT)8C	8	8	100
804	(TA)8A	6	5	83.33
805	(TA)8C	8	8	100
807	(AG)8T	8	7	87.5
808	(AG)8C	8	7	87.5
810	(GA)8T	6	6	100
811	(GA)8C	7	7	100
812	(GA)8A	6	5	83.33
814	(CT)8A	4	3	75
815	(CT)8G	5	5	100
817	(CA)8A	8	8	100
823	(TC)8C	4	4	100
832	(AT)8CTC	7	7	100
836	(AG)8CTA	6	5	83.33
842	(GA)8CTG	8	8	100
843	(CT)8AGA	8	8	100
844	(CT)8AGC	6	6	100
845	(CT)8AGG	8	8	100
847	(CA)8AGC	8	8	100
857	(AC)8CTG	9	8	88.89
859	(TG)8AGC	8	8	100
874	(CCCT)4	5	5	100
平均	--	6.74	6.43	95.48
总数	--	155	148	--

引物名称	序列(5'-3')	总位点数	多态性位点数	多态性位点百分率/%
801	(AT)8T	4	4	100
803	(AT)8C	8	8	100
804	(TA)8A	6	5	83.33
805	(TA)8C	8	8	100
807	(AG)8T	8	7	87.5
808	(AG)8C	8	7	87.5
810	(GA)8T	6	6	100
811	(GA)8C	7	7	100
812	(GA)8A	6	5	83.33
814	(CT)8A	4	3	75
815	(CT)8G	5	5	100
817	(CA)8A	8	8	100
823	(TC)8C	4	4	100
832	(AT)8CTC	7	7	100
836	(AG)8CTA	6	5	83.33
842	(GA)8CTG	8	8	100
843	(CT)8AGA	8	8	100
844	(CT)8AGC	6	6	100
845	(CT)8AGG	8	8	100
847	(CA)8AGC	8	8	100
857	(AC)8CTG	9	8	88.89
859	(TG)8AGC	8	8	100
874	(CCCT)4	5	5	100
平均	--	6.74	6.43	95.48
总数	--	155	148	--

居群	多态性位点数	多态性位点百分率/%	Na	Ne	H	I
居群	多态性位点数	多态性位点百分率/%	均值	均值	均值	均值
SC	72	46.45	1.4645±0.5004	1.3147±0.3902	0.1787±0.2079	0.2629±0.2972
SD	82	52.90	1.5290±0.5008	1.3461±0.3924	0.1978±0.2071	0.2927±0.2952
JS	73	47.10	1.4710±0.5008	1.3242±0.4008	0.1821±0.2107	0.2672±0.2997
GZ	95	61.29	1.6129±0.4887	1.4780±0.4333	0.2591±0.2224	0.3729±0.3118
RB	86	55.48	1.5548±0.4986	1.4049±0.4080	0.2265±0.2158	0.3295±0.3070
居群水平			1.5264±0.4979	1.3736±0.4049	0.2088±0.2128	0.3050±0.3022
物种水平			1.9548±0.2083	1.6161±0.3147	0.3538±0.1446	0.5234±0.1856