甜菜BvGSTU9基因的生物信息学及在镉胁迫下的表达分析

doi:10.11924/j.issn.1000-6850.casb2021-0690

中国农学通报 ›› 2021, Vol. 37 ›› Issue (36): 111-118.doi: 10.11924/j.issn.1000-6850.casb2021-0690

甜菜BvGSTU9基因的生物信息学及在镉胁迫下的表达分析

周婉婷¹^,²(), 汪曼¹^,², 周翔¹^,², 高卓¹^,²^,³, 李佳佳¹^,², 李王胜¹^,², 李思琪¹^,², 王雪倩¹^,², 王录红¹^,², 刘大丽¹^,²()

¹国家甜菜种质中期库,黑龙江大学,哈尔滨 150080
²省高校甜菜遗传育种重点实验室/现代农业与生态环境学院,黑龙江大学,哈尔滨 150080
³黑龙江省普通高等学校分子生物学重点实验室/生命科学学院,哈尔滨 150080

收稿日期:2021-07-14 修回日期:2021-08-13 出版日期:2021-12-25 发布日期:2022-02-15
通讯作者: 刘大丽
作者简介:周婉婷,女,1998年出生,浙江金华人,硕士研究生,研究方向：作物分子生物学。通信地址：150080 黑龙江省哈尔滨市南岗区学府路74号黑龙江大学现代农业与生态环境学院,Tel：13282994711,E-mail： 905428635@qq.com。
基金资助:
黑龙江省自然科学基金项目“BvHIPP24基因在能源甜菜重金属镉污染生物修复中的分子机制研究”(LH2019C057);黑龙江省高校基本科研业务费黑龙江大学专项资金项目“能源甜菜BvMPT11基因的Cd污染生物修复应答机制研究”(KJCX201920);科技部财政部国家科技资源共享服务平台项目“国家作物种质资源库甜菜分库运行服务”(NCGRC-2021-017);农作物种质资源保护与利用专项”甜菜种质资源的收集、鉴定、编目、繁种与入库(圃)保存(”1921-026);黑龙江省普通本科高等学校青年创新人才培养计划“甜菜抗旱遗传资源评价及优异基因挖掘”(UNPYSCT-2020014)

BvGSTU9 Gene in Sugar Beet: Bioinformatics and Expression Analysis Under Cadmium Stress

ZHOU Wanting¹^,²(), WANG Man¹^,², ZHOU Xiang¹^,², GAO Zhuo¹^,²^,³, LI Jiajia¹^,², LI Wangsheng¹^,², LI Siqi¹^,², WANG Xueqian¹^,², WANG Luhong¹^,², LIU Dali¹^,²()

¹National Beet Medium-term Gene Bank, Heilongjiang University, Harbin 150080
²Key Laboratory of Sugar Beet Genetics and Breeding of Heilongjiang Province/College of Modern Agriculture and Ecological Environment, Heilongjiang University, Harbin 150080
³Key Laboratory of Molecular Biology of Heilongjiang Province, School of Life Sciences, Heilongjiang University, Harbin 150080

Received:2021-07-14 Revised:2021-08-13 Online:2021-12-25 Published:2022-02-15
Contact: LIU Dali

摘要/Abstract

摘要：

为了进一步研究甜菜谷胱甘肽转移酶BvGSTU9 (LOC104894060)在重金属胁迫过程中的功能。本研究以‘780016B/12优’为实验材料,对该基因序列特征、结构、功能进行预测分析,并利用qPCR检测该基因在不同浓度镉胁迫下的表达量变化。结果显示甜菜BvGSTU9基因全长925 bp,开放阅读框675 bp,编码了由224个氨基酸组成的不稳定膜外蛋白。BvGSTU9与菠菜、藜麦的氨基酸序列相似性较高,与系统发育进化树分析结果基本相符。二级和三级结构预测表明该基因主要由α-螺旋、β-折叠、延伸链及无规则卷曲组成。qPCR显示BvGSTU9基因在不同浓度的镉胁迫下均受到不同程度的诱导,因此可以推断甜菜BvGSTU9基因无论从结构还是功能上,与镉逆境胁迫存在着一定的应答关系。研究结果也为甜菜耐重金属镉机制研究提供参考依据。

关键词: 甜菜, BvGSTU9基因, 生物信息学, 镉胁迫, 表达特性

Abstract:

To further study the function of BvGSTU9 (LOC104894060) during the heavy metal stress in sugar beet (Beta vulgaris L.), ‘780016B/12You’ was chosen as the plant material in this study to forecast and analyze the sequence characteristics, structure and function of the gene. In addition, quantitative real time polymerase chain reaction (qRT-PCR) was used to detect the changes of expression under different concentrations of cadmium stress. The results show that the whole length of BvGSTU9 gene is 925 bp, and the open reading frame is 675 bp, encoding an unstable extracellular protein composed of 224 amino acids. The amino acid sequence of BvGSTU9 is similar to that of spinach and quinoa, and it is almost consistent with the phylogenetic evolution tree analysis. The prediction of the secondary structure and the tertiary structure indicate that the gene is mainly composed of α-Spiral, β-Folding, extending chain and irregular curling. qRT-PCR analysis show that BvGSTU9 gene is induced in different degrees by different concentrations of cadmium stress. Therefore, it can be inferred that the BvGSTU9 gene in sugar beet has a certain response relationship with cadmium stress both in structure and function. The results also provide a certain reference basis for the study on the mechanism of heavy metal cadmium tolerance of sugar beet.

Key words: sugar beet, BvGSTU9 gene, bioinformatics, cadmium stress, expression characteristic

中图分类号:

S566.3

周婉婷, 汪曼, 周翔, 高卓, 李佳佳, 李王胜, 李思琪, 王雪倩, 王录红, 刘大丽. 甜菜BvGSTU9基因的生物信息学及在镉胁迫下的表达分析[J]. 中国农学通报, 2021, 37(36): 111-118.

ZHOU Wanting, WANG Man, ZHOU Xiang, GAO Zhuo, LI Jiajia, LI Wangsheng, LI Siqi, WANG Xueqian, WANG Luhong, LIU Dali. BvGSTU9 Gene in Sugar Beet: Bioinformatics and Expression Analysis Under Cadmium Stress[J]. Chinese Agricultural Science Bulletin, 2021, 37(36): 111-118.

图/表 9

图1. BvGSTU9基因编码框

图2. BvGSTU9蛋白保守功能域预测

图3. BvGSTU9与其他植物GST的氨基酸序列比对

图4. BvGSTU9和其他植物中GSTs蛋白的系统进化树

图5. 甜菜BvGSTU9基因进化树和其他植物GST基因结构未翻译的5'和3'区(UTR)、编码序列(CDS)分别用蓝色、黄色表示

图6. BvGSTU9蛋白的亲疏水性

图7. 甜菜BvGSTU9蛋白的二级结构预测

图8. 甜菜BvGSTU9蛋白的三级结构预测

图9. 甜菜BvGSTU9基因在不同浓度、不同时间梯度下的表达分析

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甜菜BvGSTU9基因的生物信息学及在镉胁迫下的表达分析

BvGSTU9 Gene in Sugar Beet: Bioinformatics and Expression Analysis Under Cadmium Stress

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