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中国农学通报 ›› 2022, Vol. 38 ›› Issue (20): 20-28.doi: 10.11924/j.issn.1000-6850.casb2021-0814

所属专题: 生物技术 玉米

• 生物科学 • 上一篇    下一篇

玉米芯降解复合菌剂的构建及其发酵效果初探

韩晓云1,2(), 胡长林1,3, 许可1,3, 郑桂华1,3, 郭宇豪1,3, 孙庆申1,3()   

  1. 1黑龙江大学 农业微生物技术教育部工程研究中心,哈尔滨 150500
    2黑龙江大学 生命科学学院 黑龙江省普通高等学校分子生物学重点实验室,哈尔滨 150080
    3黑龙江大学 生命科学学院 黑龙江省普通高等学校微生物重点实验室,哈尔滨 150080
  • 收稿日期:2021-08-24 修回日期:2021-12-05 出版日期:2022-07-15 发布日期:2022-08-23
  • 通讯作者: 孙庆申
  • 作者简介:韩晓云,女,1970年出生,黑龙江北安人,副教授,博士,主要从事食品与药品活性物质的挖掘与研发方面的研究。通信地址:150080 黑龙江省哈尔滨市南岗区学府路74号 黑龙江大学生命科学学院,Tel:0451-86609764,E-mail: hanxiaoyun70@126.com
  • 基金资助:
    黑龙江大学生物质和矿物质资源高效利用协同创新中心研发、孵化与转化支持计划

Construction of Corncob Degradation Compound Microbial Agent and Analysis of Its Degradation Effect

HAN Xiaoyun1,2(), HU Changlin1,3, XU Ke1,3, ZHENG Guihua1,3, GUO Yuhao1,3, SUN Qingshen1,3()   

  1. 1Engineering Research Center of Agricultural Microbiology Technology, Ministry of Education, Heilongjiang University, Harbin 150500
    2Key Laboratory of Molecular Biology, College of Heilongjiang Province, School of Life Sciences, Heilongjiang University, Harbin 150080
    3Key Laboratory of Microbiology, College of Heilongjiang Province, School of Life Sciences, Heilongjiang University, Harbin 150080
  • Received:2021-08-24 Revised:2021-12-05 Online:2022-07-15 Published:2022-08-23
  • Contact: SUN Qingshen

摘要:

旨在构建复合菌剂以降解玉米芯,使生物质能源得到更好的利用,并且减少环境污染。本实验以牛粪及牛粪玉米芯发酵料为试材,用刚果红染色法和滤纸酶活法(FPA)筛选纤维素降解菌,并进行菌株鉴定;将所得到的菌株通过划线法进行拮抗实验;最后将菌株复配接种到玉米芯研究其发酵效果。结果表明:在牛粪及牛粪玉米芯发酵料中获得的菌株BC-2W、BC-2Y、BC-7W、BC-7Y、BC-12Y、BC-15W、BC-15Y、B1-2、B1-11、B2-7、B2-8和B2-9 FPA酶活均在9.0 U/mL以上,接种复合菌剂的玉米芯纤维素降解率达到(55.63±2.21)%,高于接种牛粪的玉米芯纤维素降解率。红外光谱、X衍射分析和扫描电镜观察显示,接种复合菌剂的玉米芯木质纤维素降解程度均高于接种牛粪组。复合菌剂可有效降解玉米芯,在纤维素降解方面具有较大的应用潜力,可用于玉米芯的生物资源利用。

关键词: 牛粪, 玉米芯, 复合菌剂, 纤维素降解

Abstract:

The purpose of this research is to construct a compound microbial agent to degrade corncobs, use biomass energy efficiently and decrease environmental pollution. In this study, cow dung and the fermented substrate of cow dung mixed with corncob were used as tested materials. The cellulose-degrading bacteria were screened through Congo red staining and filter paper enzyme activity (FPA), and the strains were identified. The obtained strains were subjected to antagonism analysis by the streaking method. Finally, the strains were compounded and inoculated into the corncob to study the fermentation effect. The results showed that the enzyme activities of strains BC-2W, BC-2Y, BC-7W, BC-7Y, BC-12Y, BC-15W, BC-15Y, B1-2, B1-11, B2-7, B2-8 and B2-9 obtained from both kinds of fermentation substrates were all higher than 9.0 U/mL, and the cellulose degradation rate of corncob inoculated with compound microbial agent reached (55.63 ±2.21)%, which was higher than that of corncob inoculated with cow dung. Infrared spectroscopy, X-ray diffraction analysis and scanning electron microscope results showed that the degradation degree of lignocellulose in corncob inoculated with compound microbial agent was higher than that of cow dung group. The compound microbial agent could degrade corncob effectively, and it had great application prospect in cellulose degradation and utilization of biological resources of corncob.

Key words: cow dung, corncob, compound microbial agent, cellulose degradation

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