优化预处理及脱毒工艺以制备玉米芯半纤维素水解液

doi:10.11924/j.issn.1000-6850.casb20190400042

中国农学通报 ›› 2020, Vol. 36 ›› Issue (20): 28-35.doi: 10.11924/j.issn.1000-6850.casb20190400042

所属专题：玉米

优化预处理及脱毒工艺以制备玉米芯半纤维素水解液

王家旺¹^,², 杨晓峰¹^,², 刘国明¹^,², 葛菁萍¹^,²()

¹农业微生物技术教育部工程研究中心,哈尔滨 150500
²黑龙江大学生命科学学院微生物省高校重点实验室,哈尔滨 150080

收稿日期:2019-04-23 修回日期:2019-06-24 出版日期:2020-07-15 发布日期:2020-07-20
通讯作者: 葛菁萍
作者简介:王家旺,男,1995年出生,黑龙江铁力人,硕士研究生,研究方向：微生物资源挖掘与利用。通信地址：150080 黑龙江省哈尔滨市南岗区学府路74号黑龙江大学224信箱,Tel：0451-86609016,E-mail：wangjiawang2y@126.com。
基金资助:
国家自然科学基金“从2,3-丁二醇代谢角度构建工程微生物群体及其生态学机制研究”(31570492);黑龙江省教育厅重点项目“利用肺炎克雷伯氏菌发酵生产2,3-丁二醇及机理探讨”(HDJCCX-2016Z05)

Corncob Hemicellulose Hydrolysate: Optimized Pretreatment and Detoxification Process

Wang Jiawang¹^,², Yang Xiaofeng¹^,², Liu Guoming¹^,², Ge Jingping¹^,²()

¹Engineering Research Center of Agricultural Microbiology Technology, Ministry of Education, Heilongjiang University, Harbin 150500
²Key Laboratory of Microbiology, College of Heilongjiang Province, School of Life Sciences, Heilongjiang University, Harbin 150080

Received:2019-04-23 Revised:2019-06-24 Online:2020-07-15 Published:2020-07-20
Contact: Ge Jingping

摘要/Abstract

摘要：

为了降低玉米芯半纤维素水解液中的乙酸、糠醛和酚类等有毒物质含量,使可利用糖类含量增加。本研究以玉米芯为原料制备水解液,对预处理及脱毒工艺进行优化,并利用高效液相色谱(HPLC)检测优化效果。结果表明,10~20目的玉米芯在10%氨水浸泡24 h后,可将乙酸和酚类物质的脱除率,分别提高至93.4%和32.3%,而糠醛的含量也大大降低。经过真空浓缩、CaO脱毒以及2%活性炭吸附后,使有毒物质含量再次降低,此时的水解液可完全用于菌株的发酵试验,达到了去除的目的。本文所得到的最佳水解液处理步骤,其结果符合菌株发酵用标准,说明水解液处理方式的不同,直接影响着水解液的制备。这为研究和利用木质纤维素等可再生资源提供了思路,也为发酵生产奠定了理论基础。

关键词: 玉米芯半纤维素水解液, 预处理, 脱毒, 优化

Abstract:

To reduce the content of toxic substances such as acetic acid, furfural and phenols in the corncob hemicellulose hydrolyzate, and to increase the available sugar content, corncob was used as raw material to prepare hydrolysate, and the pretreatment and detoxification process were optimized, and the optimization effect was detected by high performance liquid chromatography (HPLC). The results showed that the removal rate of acetic acid and phenolic substances could be increased to 93.4% and 32.3%, respectively, after the 10~20 mesh corncob was soaked in 10% ammonia water for 24 h, and the content of furfural was also greatly reduced. After vacuum concentration, CaO detoxification and 2% activated carbon adsorption, the content of toxic substances was reduced again. At this time, the hydrolyzate could be completely used for the strain fermentation, and the purpose of removal was achieved. The optimal hydrolyzate treatment step obtained in this study could meet the standards for the fermented strain, indicating that the treatment method of hydrolyzate directly affects the preparation of hydrolyzate. The study provides an idea for the research and utilization of renewable resources such as lignocellulose, and lays a research foundation for fermentation production.

Key words: corncob hemicellulose hydrolysate, pretreatment, detoxification, optimization

中图分类号:

S216.2

王家旺, 杨晓峰, 刘国明, 葛菁萍. 优化预处理及脱毒工艺以制备玉米芯半纤维素水解液[J]. 中国农学通报, 2020, 36(20): 28-35.

Wang Jiawang, Yang Xiaofeng, Liu Guoming, Ge Jingping. Corncob Hemicellulose Hydrolysate: Optimized Pretreatment and Detoxification Process[J]. Chinese Agricultural Science Bulletin, 2020, 36(20): 28-35.

图/表 9

参考文献 35

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氨水浓度	物质种类
氨水浓度	葡萄糖	木糖	阿拉伯糖	乙酸	糠醛
0%氨水	0.24	6.22	0.36	0.41	0.037
2%氨水	0.17	1.91	0.10	0.035	0.011
5%氨水	0.20	1.53	0.49	0.033	0.002
8%氨水	0.52	3.63	0.42	0.035	0
10%氨水	0.13	5.77	0.30	0.006	0
15%氨水	0.09	3.55	0.06	0.0057	0
18%氨水	0.10	2.74	0.07	0	0

氨水浓度	物质种类
氨水浓度	葡萄糖	木糖	阿拉伯糖	乙酸	糠醛
0%氨水	0.24	6.22	0.36	0.41	0.037
2%氨水	0.17	1.91	0.10	0.035	0.011
5%氨水	0.20	1.53	0.49	0.033	0.002
8%氨水	0.52	3.63	0.42	0.035	0
10%氨水	0.13	5.77	0.30	0.006	0
15%氨水	0.09	3.55	0.06	0.0057	0
18%氨水	0.10	2.74	0.07	0	0

优化预处理及脱毒工艺以制备玉米芯半纤维素水解液

Corncob Hemicellulose Hydrolysate: Optimized Pretreatment and Detoxification Process

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氨水浓度	物质损失率（去除率）
氨水浓度	葡萄糖损失率	木糖损失率	阿拉伯糖损失率	乙酸去除率	糠醛去除率
0%氨水	41	37	50	55	68
2%氨水	27	10	33	53	56
5%氨水	27	32	13	66	51
8%氨水	29	32	16	53	83
10%氨水	33	13	16	39	76
15%氨水	37	8	14	51	100
18%氨水	35	32	18	100	100

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