BvHIPP24介导甜菜应答镉胁迫的作用分析

doi:10.11924/j.issn.1000-6850.casb2023-0080

中国农学通报 ›› 2023, Vol. 39 ›› Issue (27): 110-117.doi: 10.11924/j.issn.1000-6850.casb2023-0080

BvHIPP24介导甜菜应答镉胁迫的作用分析

黄烁淇¹^,²(), 赵晓鑫²^,³, 翟妍²^,³, 倪渊嵘²^,³, 鲁振强¹(), 刘大丽²^,³()

¹ 黑龙江大学省高校生化与分子生物学重点实验室/黑龙江大学生命科学学院，哈尔滨 150080
² 国家甜菜种质中期库，哈尔滨 150080
³ 黑龙江省普通高等学校甜菜遗传育种重点实验室/黑龙江大学现代农业与生态环境学院，哈尔滨 150080

收稿日期:2023-01-23 修回日期:2023-03-15 出版日期:2023-09-25 发布日期:2023-09-22
通讯作者: 鲁振强，男，1965年出生，吉林长春人，教授，博士，研究方向：植物分子生物学。通信地址：150080 黑龙江省哈尔滨市南岗区学府路74号黑龙江大学生命科学学院，Tel：0451-86609494，E-mail：zhenqianglu@163.com。刘大丽，女，1981年出生，辽宁盘锦人，副研究员，博士，研究方向：作物种质资源与抗性生理。通信地址：150080 黑龙江省哈尔滨市南岗区学府路74号黑龙江大学现代农业与生态环境学院，Tel：0451-86609494，E-mail：daliliu_hlju@163.com。
作者简介:
黄烁淇，女，2000年出生，黑龙江伊春人，在读研究生，研究方向：分子生物学。通信地址：150080 黑龙江省哈尔滨市南岗区学府路74号黑龙江大学，Tel：19804581853，E-mail：3294157497@qq.com。
基金资助:
黑龙江省自然科学基金项目“BvHIPP24基因在能源甜菜重金属镉污染生物修复中的分子机制研究”(LH2019C057); 甜菜种质资源精准鉴定(19230709); 普查收集甜菜种质资源鉴定评价与编目入库项目(19230801); 国家糖料产业技术体系项目“甜菜种质资源鉴定与新种质创制”(CARS-170102); 黑龙江省教育厅基本科研业务费“镉胁迫下能源甜菜金属伴侣蛋白BvHIPPs家族成员的应答机制和功能研究”(2022-KYYWF-1070)

Analysis of BvHIPP24 Involved in Sugar Beet Response to Cadmium Stress

HUANG Shuoqi¹^,²(), ZHAO Xiaoxin²^,³, ZHAI Yan²^,³, NI Yuanrong²^,³, LU Zhenqiang¹(), LIU Dali²^,³()

¹ Key Laboratory of Biochemistry and Molecular Biology/College of Life Sciences, Heilongjiang University, Harbin 150080
² National Beet Germplasm Mid-term Bank, Harbin 150080
³ Key Laboratory of Beet Genetics and Breeding/College of Modern Agriculture and Ecological Environment, Heilongjiang University, Harbin 150080

Received:2023-01-23 Revised:2023-03-15 Published-:2023-09-25 Online:2023-09-22

摘要/Abstract

摘要：

为探究甜菜重金属相关异戊二烯化植物蛋白24(BvHIPP24)在重金属镉胁迫下的作用机制，本研究利用生信方法预测了该基因上游的顺式作用元件、转录因子和下游的互作蛋白，并分析了它们在镉胁迫下的转录表达特性。结果表明，BvHIPP24含有CGTCA-motif、生长素类、光响应、MYB结合、厌氧诱导和水杨酸应答相关的作用元件；转录因子BVRB_2g046790、BVRB_1g021800和BVRB_6g128880可能参与BvHIPP24的转录调控；BvHIPP24的互作蛋白主要包括SOD、NaKR1-like、COX1、MT2、NaKR3、REP1、RCA和LHCP等，它们可能通过与重金属离子结合参与离子运输和维持细胞内环境稳态。转录组测序结果表明BvHIPP24及互作蛋白的转录表达均受到镉胁迫的调控。综上推测，BvHIPP24主要通过上游转录因子的调控表达，直接或间接的与互作蛋白共同介导镉离子螯合及解毒。本研究为进一步揭示甜菜对镉胁迫的响应机制提供了参考。

Abstract:

The aim was to explore the mechanism of heavy metal-related isoprenoid plant protein 24 (BvHIPP24) in sugar beet under cadmium stress. This study used bioinformatics methods to predict the cis-acting elements, transcription factors, and interacting proteins upstream of the gene, and analyzed their transcriptional expression characteristics under cadmium stress. The results showed that BvHIPP24 contained cis-acting elements related to CGTCA-motif, auxin response, light response, MYB binding, anaerobic induction, and salicylic acid response. Transcription factors such as BVRB_2g046790, BVRB_1g021800 and BVRB_6g128880 might be involved in the transcriptional regulation of BvHIPP24. The interaction proteins of BvHIPP24 mainly included SOD, NaKR1-like, COX1, MT2, NaKR3, REP1, RCA and LHCP, and etc., which might participate in ion transport and intracellular homeostasis by binding with heavy metal ions. Transcriptome sequencing results indicated that the transcriptional expression of BvHIPP24 and its interacting proteins were regulated by cadmium stress. Based on the above findings, BvHIPP24 was mainly expressed through the regulation of upstream transcription factors, and directly or indirectly mediated cadmium ion chelation and detoxification together with interacting proteins. This study provided a reference to further uncover the response mechanisms of sugar beets to cadmium stress.

Key words: sugar beet, heavy metal-associated isoprenylated plant protein (HIPP), cadmium stress, protein interaction, bioinformatics

黄烁淇, 赵晓鑫, 翟妍, 倪渊嵘, 鲁振强, 刘大丽. BvHIPP24介导甜菜应答镉胁迫的作用分析[J]. 中国农学通报, 2023, 39(27): 110-117.

HUANG Shuoqi, ZHAO Xiaoxin, ZHAI Yan, NI Yuanrong, LU Zhenqiang, LIU Dali. Analysis of BvHIPP24 Involved in Sugar Beet Response to Cadmium Stress[J]. Chinese Agricultural Science Bulletin, 2023, 39(27): 110-117.

图/表 5

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名称	基因ID	蛋白ID	CDS/nt	氨基酸数量	蛋白质分子量/kDa	等电点(pI)	不稳定系数	脂肪系数	亲水性指数
HIPP24	BVRB_1g021330	XP_010692589	444	147	16.378	9.39	17.54	65.65	-0.416
SOD	BVRB_9g206190	XP_010689245	687	228	23.14	5.73	22.72	91.10	0.067
SOD2	BVRB_3g068430	XP_010693815	474	157	15.82	6.86	13.35	85.03	-0.111
SOD	BVRB_4g082950	XP_010674951	459	152	15.25	5.28	22.90	75.59	-0.179
NaKR1-like	BVRB_5g103030	XP_010676785	960	319	34.51	9.76	58.34	69.91	-0.514
COX	BVRB_4g096790	XP_010694991	225	74	8.17	7.59	62.02	58.11	-0.765
MT2	BVRB_1g000270	XP_010667708	237	78	7.93	4.55	35.23	31.28	-0.253
NaKR3	BVRB_7g174670	XP_010685849	1041	346	38.54	9.66	41.28	65.29	-0.705
REP1	BVRB_8g199080	XP_010696511	1677	558	61.46	4.88	52.94	87.76	-0.160
RCA	BVRB_8g189100	XP_010687287	1359	452	50.93	6.94	38.90	89.87	-0.363
LHCP	BVRB_2g033010	XP_010669087	813	270	29.05	7.67	32.95	76.37	-0.055

名称	基因ID	蛋白ID	CDS/nt	氨基酸数量	蛋白质分子量/kDa	等电点(pI)	不稳定系数	脂肪系数	亲水性指数
HIPP24	BVRB_1g021330	XP_010692589	444	147	16.378	9.39	17.54	65.65	-0.416
SOD	BVRB_9g206190	XP_010689245	687	228	23.14	5.73	22.72	91.10	0.067
SOD2	BVRB_3g068430	XP_010693815	474	157	15.82	6.86	13.35	85.03	-0.111
SOD	BVRB_4g082950	XP_010674951	459	152	15.25	5.28	22.90	75.59	-0.179
NaKR1-like	BVRB_5g103030	XP_010676785	960	319	34.51	9.76	58.34	69.91	-0.514
COX	BVRB_4g096790	XP_010694991	225	74	8.17	7.59	62.02	58.11	-0.765
MT2	BVRB_1g000270	XP_010667708	237	78	7.93	4.55	35.23	31.28	-0.253
NaKR3	BVRB_7g174670	XP_010685849	1041	346	38.54	9.66	41.28	65.29	-0.705
REP1	BVRB_8g199080	XP_010696511	1677	558	61.46	4.88	52.94	87.76	-0.160
RCA	BVRB_8g189100	XP_010687287	1359	452	50.93	6.94	38.90	89.87	-0.363
LHCP	BVRB_2g033010	XP_010669087	813	270	29.05	7.67	32.95	76.37	-0.055

BvHIPP24介导甜菜应答镉胁迫的作用分析

Analysis of BvHIPP24 Involved in Sugar Beet Response to Cadmium Stress

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