水稻锌稳态调控基因和分子机制的研究进展

doi:10.11924/j.issn.1000-6850.casb2022-0891

中国农学通报 ›› 2023, Vol. 39 ›› Issue (32): 22-32.doi: 10.11924/j.issn.1000-6850.casb2022-0891

水稻锌稳态调控基因和分子机制的研究进展

李荣田¹(), 李双语燕¹^,², 孟丽君², 刘长华¹^,³(), 詹俊辉²()

¹ 黑龙江大学生命科学学院农业微生物技术教育部工程研究中心/黑龙江省寒区植物基因与生物发酵重点实验室/黑龙江省普通高校分子生物学重点实验室，哈尔滨 150080
² 中国农业科学院深圳农业基因组研究所，广东深圳 518120
³ 黑龙江大学现代农业与生态环境学院，哈尔滨 150080

收稿日期:2023-02-21 修回日期:2023-04-18 出版日期:2023-11-15 发布日期:2023-11-10
通讯作者: 刘长华，女，1970年出生，黑龙江人，副教授，博士，研究方向：水稻分子育种与分子生物学。通信地址：150080 黑龙江省哈尔滨市南岗区学府路74号黑龙江大学现代农业与生态环境学院，Tel：0451-86608243，E-mail：liuchanghua70@163.com；詹俊辉，男，1993年出生，河南淮滨人，博士，研究方向：水稻遗传育种与分子生物学。通信地址：518120 广东省深圳市大鹏新区布新路97号中国农业科学院深圳农业基因组研究所，E-mail：zhanjunhui@caas.cn。
作者简介:
李荣田，男，1963年出生，吉林德惠人，教授，博士，研究方向：水稻分子育种与分子生物学。通信地址：150080 黑龙江省哈尔滨市南岗区学府路74号黑龙江大学生命科学技术学院，Tel：0451-86608243，E-mail：lirongtian07@aliyun.com。
基金资助:
中央引导地方科技发展基金“三江稻区绿色优质水稻品种及其双减栽培体系示范推广”(ZYYD2022JMS004)

Research Progress of Zinc Homeostasis Related Genes and Molecular Mechanism in Rice

LI Rongtian¹(), LI Shuangyuyan¹^,², MENG Lijun², LIU Changhua¹^,³(), ZHAN Junhui²()

¹ Engineering Research Center of Agricultural Microbiology Technology, Ministry of Education/ Heilongjiang Provincial Key Laboratory of Plant Genetic Engineering and Biological Fermentation Engineering for Cold Region/ Key Laboratory of Molecular Biology in Colleges of Heilongjiang Province/School of Life Sciences, Heilongjiang University, Harbin 150080
² CAAS-IRRI Joint Laboratory for Genomics-Assisted Germplasm Enhancement, Agricultural Genomics Institute at Shenzhen, Chinese Academy of Agricultural Sciences, Shenzhen, Guangdong 518120
³ College of Advanced Agriculture and Ecological Environment, Heilongjiang University, Harbin 150080

Received:2023-02-21 Revised:2023-04-18 Published-:2023-11-15 Online:2023-11-10

摘要/Abstract

摘要：

锌(Zn)是动物植物所必需的微量营养素，缺锌或锌过量会严重影响水稻的生长发育。水稻锌含量维持在一定范围有助于提高其产量和品质，并且可以提高籽粒的锌含量，一定程度上可以解决目前人体缺锌的问题。因此研究水稻锌的吸收、转运和分配，和其他调控锌稳态分子机制至关重要。基于近年来国内外研究报道，简要概述了锌在植物体内的重要性，重点介绍了水稻中的离子转运体和目前已经报道的分子机制。总结了这些离子转运体参与了锌从土壤的吸收，从根部向地上部的转运，以及分配到水稻各部位，还总结了一些和离子转运体相关的分子机制。以期为水稻锌稳态调控基因的挖掘、分子机制的研究和富锌水稻种质的创制提供参考。

关键词: 水稻, 锌稳态, 转运蛋白, 分子机制, 研究进展

Abstract:

Zinc (Zn) is an essential micronutrient for animals and plants. Zinc deficiency or excess can seriously affect the growth and development of rice. Maintaining zinc content in rice at a certain level is helpful to improve the yield and quality of rice, increase the zinc content in grain, and solve the current problem of zinc deficiency in human body to a certain extent. Therefore, it is important to understand Zn uptake, transport, distribution, and other molecular mechanisms regulating Zn homeostasis in rice. In this review, we briefly summarized the importance of zinc in plants, especially the ion transporters in rice and the molecular mechanisms. The roles of these ion transporters in the uptake of Zn from soil, the transport from root to shoot, and the distribution of Zn to various parts of rice were summarized. Some molecular mechanisms related to ion transporters were also summarized. This study provides reference for the mining of zinc homeostasis regulatory genes in rice, the study of molecular mechanism, and the creation of high zinc rice germplasm.

Key words: rice, zinc homeostasis, zinc transporter, molecular mechanism, research progress

李荣田, 李双语燕, 孟丽君, 刘长华, 詹俊辉. 水稻锌稳态调控基因和分子机制的研究进展[J]. 中国农学通报, 2023, 39(32): 22-32.

LI Rongtian, LI Shuangyuyan, MENG Lijun, LIU Changhua, ZHAN Junhui. Research Progress of Zinc Homeostasis Related Genes and Molecular Mechanism in Rice[J]. Chinese Agricultural Science Bulletin, 2023, 39(32): 22-32.

图/表 4

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基因名称	基因序列号	表达部位	亚细胞定位	在酵母中锌转运活性	锌功能	参考文献
OsZIP1	LOC_Os01g74110	根、茎	内质网膜和质膜	否	吸收锌	[45]
OsZIP3	LOC_Os04g52310	节点	质膜	是	分配锌	[56]
OsZIP4	LOC_Os08g10630	根、茎	质膜	是	转运和分配锌	[53,57]
OsZIP5	LOC_Os05g39560	根	质膜	是	吸收锌	[49-50]
OsZIP7	LOC_Os05g10940	根、节点	质膜	是	转运和分配锌	[55,58]
OsZIP8	LOC_Os07g12890	根、茎	质膜	是	分配锌	[59]
OsZIP9	LOC_Os05g39540	根	质膜	是	吸收锌	[48,50]
OsIRT1	LOC_Os03g46470	根	质膜	是	吸收锌	[51,52]
OsHMA1	LOC_Os06g47550	根和茎	质体	-	-	[64-65]
OsHMA2	LOC_Os06g48720	根和节点	细胞质膜	-	转运锌	[66-67]
OsHMA3	LOC_Os07g12900	根和节点	液泡膜	-	转运锌	[68]
OsHMA9	LOC_Os06g45500	维管束和花药	细胞质膜	-	转运锌	[69]
OsNAS1	LOC_Os03g19427	根	根的伴胞和原生木质部中柱鞘	-	-	[71-72]
OsNAS2	LOC_Os03g19420	根	根的伴胞和原生木质部中柱鞘	-	-	[71-72]
OsNAS3	LOC_Os07g48980	叶	根的中柱鞘和伴胞，叶片的伴胞	-	-	[71-72]
OsNAAT1	LOC_Os02g20360	根和茎	原生木质部附近的伴胞和表皮细胞	-	-	[71-72]
OsMTP1	LOC_Os05g03780	叶片和茎	液泡膜	是	-	[74-75]
OsVIT1	LOC_Os04g38940	叶片	液泡膜	-	跨液泡膜运输锌	[75]
OsVIT2	LOC_Os09g23300	叶鞘	液泡膜	-	跨液泡膜运输锌	[76⇓-78]
OsZIFL12	LOC_Os12g03899	节1	液泡膜	-	分配锌	[81-82]

基因名称	基因序列号	表达部位	亚细胞定位	在酵母中锌转运活性	锌功能	参考文献
OsZIP1	LOC_Os01g74110	根、茎	内质网膜和质膜	否	吸收锌	[45]
OsZIP3	LOC_Os04g52310	节点	质膜	是	分配锌	[56]
OsZIP4	LOC_Os08g10630	根、茎	质膜	是	转运和分配锌	[53,57]
OsZIP5	LOC_Os05g39560	根	质膜	是	吸收锌	[49-50]
OsZIP7	LOC_Os05g10940	根、节点	质膜	是	转运和分配锌	[55,58]
OsZIP8	LOC_Os07g12890	根、茎	质膜	是	分配锌	[59]
OsZIP9	LOC_Os05g39540	根	质膜	是	吸收锌	[48,50]
OsIRT1	LOC_Os03g46470	根	质膜	是	吸收锌	[51,52]
OsHMA1	LOC_Os06g47550	根和茎	质体	-	-	[64-65]
OsHMA2	LOC_Os06g48720	根和节点	细胞质膜	-	转运锌	[66-67]
OsHMA3	LOC_Os07g12900	根和节点	液泡膜	-	转运锌	[68]
OsHMA9	LOC_Os06g45500	维管束和花药	细胞质膜	-	转运锌	[69]
OsNAS1	LOC_Os03g19427	根	根的伴胞和原生木质部中柱鞘	-	-	[71-72]
OsNAS2	LOC_Os03g19420	根	根的伴胞和原生木质部中柱鞘	-	-	[71-72]
OsNAS3	LOC_Os07g48980	叶	根的中柱鞘和伴胞，叶片的伴胞	-	-	[71-72]
OsNAAT1	LOC_Os02g20360	根和茎	原生木质部附近的伴胞和表皮细胞	-	-	[71-72]
OsMTP1	LOC_Os05g03780	叶片和茎	液泡膜	是	-	[74-75]
OsVIT1	LOC_Os04g38940	叶片	液泡膜	-	跨液泡膜运输锌	[75]
OsVIT2	LOC_Os09g23300	叶鞘	液泡膜	-	跨液泡膜运输锌	[76⇓-78]
OsZIFL12	LOC_Os12g03899	节1	液泡膜	-	分配锌	[81-82]

基因名称	基因序列号	ZDRE元件数量	ZDRE元件	元件位置^*	碱基替换数量
OsZIP3	LOC_Os04g52310	1	GTGTCGCCTT	-1388	2
OsZIP4	LOC_Os08g10630	1	ACATCGACAC	-568	2
OsZIP5	LOC_Os05g39560	2	ATGTCGACAC	-790	0
OsZIP5	LOC_Os05g39560	2	GAATCGACAT	-480	2
OsZIP7	LOC_Os05g10940	2	ATGTCGACAC	-631	0
OsZIP7	LOC_Os05g10940	2	GTGACGACAT	-288	1
OsZIP8	LOC_Os07g12890	3	ATGTCAACAC	-283	1
			GTGTCGTCAT	-260	1
			GTGTCGTCAC	-154	1
OsZIP9	LOC_Os05g39540	2	ATGGCGACAT	-2027	1
OsZIP9	LOC_Os05g39540	2	ATGTCGACAC	-1772	0
OsZIP10	LOC_Os06g37010	3	GTGTCGACAC	-704	0
			ATGACGACAT	-485	1
			ATGACGACAT	-455	1
OsHMA1	LOC_Os06g47550	4	ATGTCGAACC	-570	2
			GTGTCGGAGC	-693	3
			GGTTCGACAC	-2753	2
			GTGACGACAC	-2764	1
OsHMA3	LOC_Os07g12900	1	ATTGCGACAT	-1354	2
OsHMA9	LOC_Os06g45500	1	GTGTCGTCTT	-1085	2
OsNAS1	LOC_Os03g19427	2	GTGTCGCTCC	-1887	3
OsNAS1	LOC_Os03g19427	2	ATACCGACAC	-2404	2
OsNAS2	LOC_Os03g19420	3	ATACCGACAC	-720	2
			GTGTCTAAAT	-486	2
			GTGTCGCTCC	-1227	3
OsNAS3	LOC_Os07g48980	1	ATGCTGACAT	-1767	2
OsMTP1	LOC_Os05g03780	1	AACACGACAT	-1078	3
OsVIT1	LOC_Os04g38940	3	ATTTGGACAT	-1211	2
			GTGTCGGTCT	-1847	3
			GTAGAGACAT	-1902	3
OsVIT2	LOC_Os09g23300	1	GTGCAGACAT	-2407	2
OsZIFL12	LOC_Os12g03899	2	GTGTCGCCGT	-2426	2
OsZIFL12	LOC_Os12g03899	2	AAACCGACAT	-2922	3
OsFRDL1	LOC_Os03g11734	1	GTGTCGTTGT	-1296	3

基因名称	基因序列号	ZDRE元件数量	ZDRE元件	元件位置^*	碱基替换数量
OsZIP3	LOC_Os04g52310	1	GTGTCGCCTT	-1388	2
OsZIP4	LOC_Os08g10630	1	ACATCGACAC	-568	2
OsZIP5	LOC_Os05g39560	2	ATGTCGACAC	-790	0
OsZIP5	LOC_Os05g39560	2	GAATCGACAT	-480	2
OsZIP7	LOC_Os05g10940	2	ATGTCGACAC	-631	0
OsZIP7	LOC_Os05g10940	2	GTGACGACAT	-288	1
OsZIP8	LOC_Os07g12890	3	ATGTCAACAC	-283	1
			GTGTCGTCAT	-260	1
			GTGTCGTCAC	-154	1
OsZIP9	LOC_Os05g39540	2	ATGGCGACAT	-2027	1
OsZIP9	LOC_Os05g39540	2	ATGTCGACAC	-1772	0
OsZIP10	LOC_Os06g37010	3	GTGTCGACAC	-704	0
			ATGACGACAT	-485	1
			ATGACGACAT	-455	1
OsHMA1	LOC_Os06g47550	4	ATGTCGAACC	-570	2
			GTGTCGGAGC	-693	3
			GGTTCGACAC	-2753	2
			GTGACGACAC	-2764	1
OsHMA3	LOC_Os07g12900	1	ATTGCGACAT	-1354	2
OsHMA9	LOC_Os06g45500	1	GTGTCGTCTT	-1085	2
OsNAS1	LOC_Os03g19427	2	GTGTCGCTCC	-1887	3
OsNAS1	LOC_Os03g19427	2	ATACCGACAC	-2404	2
OsNAS2	LOC_Os03g19420	3	ATACCGACAC	-720	2
			GTGTCTAAAT	-486	2
			GTGTCGCTCC	-1227	3
OsNAS3	LOC_Os07g48980	1	ATGCTGACAT	-1767	2
OsMTP1	LOC_Os05g03780	1	AACACGACAT	-1078	3
OsVIT1	LOC_Os04g38940	3	ATTTGGACAT	-1211	2
			GTGTCGGTCT	-1847	3
			GTAGAGACAT	-1902	3
OsVIT2	LOC_Os09g23300	1	GTGCAGACAT	-2407	2
OsZIFL12	LOC_Os12g03899	2	GTGTCGCCGT	-2426	2
OsZIFL12	LOC_Os12g03899	2	AAACCGACAT	-2922	3
OsFRDL1	LOC_Os03g11734	1	GTGTCGTTGT	-1296	3

基因名称	基因序列号	RR元件(AGATT)	基因名称	基因序列号	RR元件(AGATT)	基因名称	基因序列号	RR元件(AGATT)
OsZIP1	LOC_Os01g74110	2	OsZIP11	LOC_Os05g25194	6	OsHMA9	LOC_Os06g45500	5
OsZIP2	LOC_Os03g29850	5	OsZIP13	LOC_Os02g10230	7	OsNAS1	LOC_Os03g19427	3
OsZIP3	LOC_Os04g52310	8	OsZIP14	LOC_Os08g36420	2	OsNAS3	LOC_Os07g48980	5
OsZIP4	LOC_Os08g10630	3	OsZIP16	LOC_Os08g01030	3	OsVIT1	LOC_Os04g38940	1
OsZIP5	LOC_Os05g39560	4	OsIRT1	LOC_Os03g46470	3	OsVIT2	LOC_Os09g23300	7
OsZIP6	LOC_Os05g07210	3	OsIRT2	LOC_Os03g46454	4	OsNAAT1	LOC_Os02g20360	4
OsZIP7	LOC_Os05g10940	2	OsHMA1	LOC_Os06g47550	2	OsMTP1	LOC_Os05g03780	5
OsZIP8	LOC_Os07g12890	1	OsHMA2	LOC_Os06g48720	8	OsZIFL12	LOC_Os12g03899	4
OsZIP9	LOC_Os05g39540	8	OsHMA3	LOC_Os07g12900	5	OsFRDL1	LOC_Os03g11734	3
OsZIP10	LOC_Os06g37010	2

水稻锌稳态调控基因和分子机制的研究进展

Research Progress of Zinc Homeostasis Related Genes and Molecular Mechanism in Rice

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