硒对硼毒害下萝卜幼苗生理特性的影响

doi:10.11924/j.issn.1000-6850.casb2023-0475

中国农学通报 ›› 2024, Vol. 40 ›› Issue (16): 36-42.doi: 10.11924/j.issn.1000-6850.casb2023-0475

硒对硼毒害下萝卜幼苗生理特性的影响

袁伟玲¹(), 陈卫芳¹, 刘志雄¹, 陈磊夫¹(), 吴润²()

¹ 湖北省农业科学院经济作物研究所/蔬菜种质创新与遗传改良湖北省重点实验室，武汉 430064
² 湖北省耕地质量与肥料工作总站，武汉 430070

收稿日期:2023-06-29 修回日期:2024-01-11 出版日期:2024-06-05 发布日期:2024-05-30
通讯作者:
陈磊夫，男，1981年出生，湖北黄石人，副研究员，博士，主要从事蔬菜营养方面的研究。通信地址：430064 武汉市洪山区南湖大道43号湖北省农业科学院经济作物研究所，Tel：027-87389436，E-mail：chenleifu2018@163.com。
吴润，女，1975年出生，湖北京山人，高级农艺师，学士，主要从事蔬菜肥料方面的研究。通信地址：430070 湖北省武汉市洪山区珞狮路362，Tel：027-87396623，E-mail：735650793@qq.com。
作者简介:
袁伟玲，女，1977年出生，湖北随州人，研究员，博士，主要从事萝卜营养与品质方面的研究。通信地址：430064 武汉市洪山区南湖大道43号湖北省农业科学院经济作物研究所，Tel：027-87389436，E-mail：ywling2012@126.com。
基金资助:
湖北省科技创新专项重大项目“蔬菜新品种选育及健康栽培技术研究与示范”(2019-620-000-001-07); 湖北省农业科技创新中心创新团队(2021-620-000-001-01); 现代农业产业技术体系建设专项资金(CARS-25-G-29)

Effect of Selenium on Physiological Characteristics of Radish Seedlings Under Boron Toxicity

YUAN Weiling¹(), CHEN Weifang¹, LIU Zhixiong¹, CHEN Leifu¹(), WU Run²()

¹ Institute of Economic Crop, Hubei Academy of Agricultural Science, Wuhan 430064
² Hubei Provincial General Station of Cultivated Land Quality and Fertilizer, Wuhan 430070

Received:2023-06-29 Revised:2024-01-11 Published:2024-06-05 Online:2024-05-30

摘要/Abstract

摘要：

在明确不同浓度硒和硼对萝卜幼苗生长影响的基础上，进一步探索硒对硼毒害下萝卜幼苗生物量及活性氧代谢的影响，旨在为减轻萝卜幼苗硼毒害提供技术支撑。以‘雪单1号’萝卜为试验材料，采用盆栽试验，设置5个硒浓度(0、2.5、5、7.5、10 μmol/L)和5个硼浓度(0、125、250、500、750 μmol/L)，研究叶面喷施不同浓度的硒和硼对萝卜幼苗生长的影响；在此基础上，选取2个硼处理(250、500 μmol/L)，通过叶面喷施清水和适量硒(5 μmol/L)，研究适量硒对硼毒害下萝卜幼苗生理特性的影响。结果表明，萝卜幼苗鲜重随叶面喷施硒和硼浓度的增加呈现先增加后降低的趋势，萝卜幼苗适宜的硒喷施浓度为5 μmol/L、叶面喷施硼浓度为500 μmol/L时，萝卜幼苗出现中毒症状；与正常施硼(250 μmol/L)相比，硼毒害(500 μmol/L)显著降低了萝卜幼苗干物质重，增加了硼含量和硼累积量，降低萝卜幼苗过氧化氢酶(CAT)、过氧化物酶(POD)、抗坏血酸过氧化物酶(APX)和谷胱甘肽(GSH)的含量，增加过氧化氢(H₂O₂)和丙二醛(MDA)含量。叶面喷施适量硒(5 μmol/L)，与无硒处理相比，硼毒害处理萝卜幼苗硼含量、硼累积量分别降低40.06%、37.36%，幼苗POD、CAT、APX抗氧化酶活性分别增加14.82%、17.45%、18.05%，非酶抗氧化物GSH、抗坏血酸(ASA)含量分别增加13.89%、5.88%，H₂O₂、MDA含量分别降低28.43%、20.45%。适量硒可以提高萝卜幼苗抗氧化作用，降低活性氧累积，减轻过量硼对萝卜幼苗产生的毒害，扩大植株对硼的适应范围。

关键词: 萝卜, 硒, 硼毒害, 抗氧化酶活性, 非酶抗氧化物含量

Abstract:

This paper aims to investigate the effects of different concentrations of selenium and boron on the growth of radish seedlings, further explore the effects of selenium on the biomass and reactive oxygen species metabolism of radish seedlings under boron toxicity, providing technical support for reducing boron toxicity in radish seedlings. Using radish seedlings (Raphanus sativus ‘Xuedan No.1’) as the test material, a pot experiment was conducted with 5 boron concentrations (0, 125, 250, 500, 750 μmol/L) and 5 selenium concentrations (0, 2.5, 5, 7.5, 10 μmol/L), to study the effects of foliar spraying of different concentrations of selenium and boron on the growth of radish seedlings. Furtherly, 2 boron treatments (250 and 500 μmol/L) were sprayed with Se (0, 5 μmol/L) to study the effect of moderate selenium on physiological characteristics of radish seedlings under boron toxicity. The fresh weight of radish seedlings showed a trend of first increasing and then decreasing with the increase of selenium and boron concentrations sprayed on the leaves. When selenium spraying concentration for radish seedlings was 5 μmol/L, foliar boron spraying concentration was 500 μmol/L, radish seedlings showed symptoms of poisoning. Compared with normal boron application (250 μmol/L), boron toxicity (500 μmol/L) significantly reduced the dry matter weight of radish seedlings, increased the boron content and boron accumulation, decreased the contents of catalase (CAT), peroxidase (POD), ascorbate peroxidase (APX) and glutathione (GSH) in leaves of radish seedlings, and increased the contents of hydrogen peroxide (H₂O₂) and malondialdehyde (MDA). Spraying proper amount of selenium (5 μmol/L), compared with selenium free treatment, the boron content and boron accumulation of radish seedlings under boron toxicity treatment decreased by 40.06% and 37.36% respectively, the activities of POD, CAT, APX antioxidant enzymes in the leaves of seedlings increased by 14.82% and 17.45% respectively, the contents of non enzymatic antioxidants GSH and ascorbic acid (ASA) increased by 13.89% and 5.88% respectively, and the contents of H₂O₂ and MDA decreased by 28.43% and 20.45% respectively. The proper selenium can improve the antioxidant effect of radish seedlings, reduce the accumulation of active oxygen, reduce the toxicity of boron toxicity on radish seedlings, and improve the adaptation range of plants to boron.

Key words: radish, selenium, boron toxicity, antioxidant enzyme activity, non enzymatic antioxidant content

袁伟玲, 陈卫芳, 刘志雄, 陈磊夫, 吴润. 硒对硼毒害下萝卜幼苗生理特性的影响[J]. 中国农学通报, 2024, 40(16): 36-42.

YUAN Weiling, CHEN Weifang, LIU Zhixiong, CHEN Leifu, WU Run. Effect of Selenium on Physiological Characteristics of Radish Seedlings Under Boron Toxicity[J]. Chinese Agricultural Science Bulletin, 2024, 40(16): 36-42.

图/表 6

参考文献 36

[1]	YAU S K, RYAN J. Boron toxicity tolerance in crops: A viable alternative to soil amelioration[J]. Crop science, 2008, 48:854-865.
[2]	王春利, 邢小茹, 吴国平, 等. 宽甸土壤及部分农作物中硼的分布及污染分析[J]. 中国环境监测, 2003, 19(5):4-7.
[3]	段碧辉, 刘新伟, 矫威, 等. 硒减轻油菜幼苗硼毒害机理的研究[J]. 中国农业科学, 2014, 47(11):2126-2134. doi: 10.3864/j.issn.0578-1752.2014.11.006
[4]	穆婷婷, 杜慧玲, 张福耀, 等. 外源硒对谷子生理特性、硒含量及其产量和品质的影响[J]. 中国农业科学, 2017, 50(1):51-63. doi: 10.3864/j.issn.0578-1752.2017.01.005
[5]	雷红量, 丛文宇, 蔡照磊, 等. 植物根系与叶片吸收硒的关键过程及影响因素[J]. 植物营养与肥料学报, 2021, 27(8):1456-1467.
[6]	YAO X Q, CHU J Z, WANG G Y. Effects of selenium on wheat seedlings under drought stress[J]. Biological trace element research, 2009(3):283-290. doi: 10.1007/s12011-009-8328-7 pmid: 19214397
[7]	HAWRYLAK-NOWAK B. Beneficial effects of exogenous selenium in cucumber seedlings subjected to salt stress[J]. Biological trace element research, 2009, 132:259-269.
[8]	CHU J, YAO X, ZHANG Z. Responses of wheat seedlings to exogenous selenium supply under cold stress[J]. Biological trace element research, 2010, 136:355-363. doi: 10.1007/s12011-009-8542-3 pmid: 19830391
[9]	周健, 郝苗, 刘永红, 等. 不同价态硒缓解小油菜镉胁迫的生理机制[J]. 植物营养与肥料学报, 2017, 23(2):444-450.
[10]	吴永尧, 卢向阳, 彭振坤, 等. 硒在水稻中的生理生化作用探讨[J]. 中国农业科学, 2000, 33(1):100-103. doi: 10.3864/j.issn.0578-1752.2000-33-1-103-106
[11]	汪隆植, 何启伟. 中国萝卜[M]. 北京: 科学技术文献出版社, 2005:1.
[12]	高慧雅, 张爱军, 赵丽. 叶面喷施锌硒肥对谷子抗氧化酶活性及籽粒锌硒含量的影响[J]. 植物营养与肥料学报, 2020, 26(9):1724-1731.
[13]	QUINTANILLA-GUERRERO F, DUARTE-VÁZQUEZ M A, GARCÍA-ALMENDAREZ B E, et al. Polyethylene glycol improves phenol removal by immobilized turnip peroxidase[J]. Bioresource technology, 2008, 99(18):8605-8611.
[14]	AEBI H. Catalase in vitro[A].// PACKER L. Methods in enzymology[M]. London: Elsevier Academic Press, 1984:121-126.
[15]	NAKANO Y, ASADA K. Hydrogen peroxide scavenged by ascorbate-specific peroxidase in spinach chloroplasts[J]. Plant cell physiology, 1981, 22:867-880.
[16]	PARADISO A, BERARDINO R, DE PINTO M C, et al. Increase in ascorbate- glutathione metabolism as local and precocious systemic responses induced by cadmium in durum wheat plants[J]. Plant cell physiology, 2008, 49:362-374.
[17]	MUKHERJEE S P, CHOUDHURI M A. Implications of water stress induced changes in the levels of endogenous ascorbic acid and hydrogen peroxide in vigna seedlings[J]. Plant physiology, 1983, 58:166-170.
[18]	CHAKRABARTY D, DATTA S K. Micropropagation of gerbera: Lipid peroxidation and antioxidant enzyme activities during acclimatization process[J]. Acta physiology plant, 2008, 30(3):325-331.
[19]	KUMAR G N M, KNOWLES N R. Changes in lipid peroxidation and lipolytic and free-radical scavenging enzyme activities during aging and sprouting of potato (Solanum tuberosum) seed-tubers[J]. Plant physiology, 1993, 102(1):115-124. doi: 10.1104/pp.102.1.115 pmid: 12231802
[20]	刘桂东, 姜存仓, 王运华, 等. 硼对两种不同砧木‘纽荷尔’脐橙叶片硼形态影响的差异[J]. 中国农业科学, 2011, 44(5):982-989.
[21]	CERVILLA L M, BLASCO B, RÍOS J J, et al. Oxidative stress and antioxidants in tomato (Solanum lycopersicum) plants subjected to boron toxicity[J]. Annals of botany, 2007, 100(4):747-756. doi: 10.1093/aob/mcm156 pmid: 17660516
[22]	ALPASLAN M, GUNES A. Interactive effects of boron and salinity stress on the growth, membrane permeability and mineral composition of tomato and cucumber plants[J]. Plant and soil, 2001, 236(1):123-128.
[23]	MARIA F, RŰCKA K, HELINA H, et al. The protective role of selenium in rape seedlings subjected to cadmium stress[J]. Journal of plant physiology, 2008(8):833-844. doi: 10.1016/j.jplph.2007.06.006 pmid: 17913288
[24]	JAHID A M, SHILPA G, NAVENEET K, et al. Selenium antagonises the toxic effects of arsenic on mungbean (Phaseolus aureus Roxb.) plants by restricting its uptake and enhancing the antioxidative and detoxification mechanisms[J]. Environmental and experimental botany, 2012, 77:242-248.
[25]	王道波. 富硒蔬菜高效栽培与深加工关键技术研究[M]. 长春: 东北师范大学出版社, 2019:2.
[26]	铁梅, 刘阳, 李华为, 等. 硒镉处理对萝卜硒镉吸收的影响及其交互作用[J]. 生态学杂志, 2014, 33(6):1587-1593.
[27]	华岩. 硒——生命的营养素[M]. 北京: 北京大学出版社, 2019:250.
[28]	赵竹青, 刘同仇, 邓波儿. 过量有效硼对莴苣、辣椒活性氧代谢的影响[J]. 华中农业大学学报, 1991, 10(3):263-267.
[29]	INAL A, PILBEAM D, GUNES A. Silicon increases tolerance to boron toxicity and reduces oxidative damage in barley[J]. Journal of plant nutrition, 2009, 32(1/3):112-128
[30]	MITTLER R. Oxidative stress, antioxidants and stress tolerance[J]. Trends in plant science, 2002, 7(9):405-410. doi: 10.1016/s1360-1385(02)02312-9 pmid: 12234732
[31]	GUNES A, INAL A, BAGCI E G, et al. Silicon increases boron tolerance and reduces oxidative damage of wheat grown in soil with excess boron[J]. Biologia plantarum, 2007, 51:571-574.
[32]	AFTAB T, KHAN M M A, IDREES M, et al. Boron induced oxidative stress, antioxidant defence response and changes in artemisinin content in Artemisia annua L.[J]. Agronomy and crop science, 2010, 196(3):423-430.
[33]	徐芳森, 刘同仇, 刘景福. 作物对过量硼毒害抗性机理的探讨[J]. 华中农业大学学报, 1993, 12(4):353-357.
[34]	KUMAR M, BIJO A J, BAGHEL R S, et al. Selenium and spermine alleviate cadmium induced toxicity in the red seaweed Gracilaria dura by regulating antioxidants and DNA methylation[J]. Plant physiology and biochemistry, 2012(51):129-138.
[35]	贾玮, 吴隽, 屈婵娟, 等. 硒增强植物抗逆能力及其机理研究进展[J], 中国农学通报, 2015, 31(14):171-176. doi: 10.11924/j.issn.1000-6850.casb14100137
[36]	SOBOLEV D, BEGONIA M F T. Effects of heavy metal contamination upon soil microbes: Lead-induced changes in general and denitrifying microbial communities as evidenced by molecular markers[J]. Environmental research and public health, 2008, 5(5):450-456.

处理	处理浓度/(μmol/L)	产量/(g/盆)	喷施后萝卜生长状况
硒	0	62.5±3.8a	萝卜幼苗生长正常
	2.5	64.2±2.3a	萝卜幼苗生长正常，无中毒症状
	5	66.6±2.5a	萝卜幼苗生长正常，无中毒症状
	7.5	56.1±2.4b	喷施后叶片发黄，之后很快恢复
	10	54.5±2.9b	喷施后叶片枯死，出现中毒症状
硼	0	62.5±3.8a	萝卜幼苗叶面较小
	125	63.1±2.6a	萝卜幼苗生长正常，无中毒症状
	250	64.6±2.2a	萝卜幼苗生长正常，无中毒症状
	500	55.9±1.8b	喷施后30%叶面枯死，出现中毒症状
	750	50.3±2.6b	喷施后70%叶面枯死，出现严重中毒症状

处理	处理浓度/(μmol/L)	产量/(g/盆)	喷施后萝卜生长状况
硒	0	62.5±3.8a	萝卜幼苗生长正常
	2.5	64.2±2.3a	萝卜幼苗生长正常，无中毒症状
	5	66.6±2.5a	萝卜幼苗生长正常，无中毒症状
	7.5	56.1±2.4b	喷施后叶片发黄，之后很快恢复
	10	54.5±2.9b	喷施后叶片枯死，出现中毒症状
硼	0	62.5±3.8a	萝卜幼苗叶面较小
	125	63.1±2.6a	萝卜幼苗生长正常，无中毒症状
	250	64.6±2.2a	萝卜幼苗生长正常，无中毒症状
	500	55.9±1.8b	喷施后30%叶面枯死，出现中毒症状
	750	50.3±2.6b	喷施后70%叶面枯死，出现严重中毒症状

硒对硼毒害下萝卜幼苗生理特性的影响

Effect of Selenium on Physiological Characteristics of Radish Seedlings Under Boron Toxicity

RichHTML

PDF (PC)

可视化

摘要/Abstract

引用本文

使用本文

图/表 6

参考文献 36

相关文章 15

编辑推荐

Metrics

本文评价

关于本刊

作者中心

审者中心

在线期刊

联系我们

[1]	杨利娟, 高苇, 李海燕, 薛涛, 张春祥, 王勇. 萝卜黑腐病的病原鉴定及室内药剂筛选[J]. 中国农学通报, 2024, 40(7): 129-134.
[2]	葛万晴, 冯棣, 张金磊, 孙小桉, 王志和, 祝海燕. 种植环境及密度对水果青萝卜生长、产量和品质的影响[J]. 中国农学通报, 2024, 40(4): 67-73.
[3]	董洁, 火顺利, 赵双印, 叶远荣, 蔡恩格力, 赵禹. 地源硒水平对加工番茄品质的影响[J]. 中国农学通报, 2024, 40(13): 36-41.
[4]	王丽, 蒋亚, 刘桂华, 柴冠群, 范成五. 不同生育期喷施硒肥对白菜硒镉吸收影响研究[J]. 中国农学通报, 2024, 40(11): 44-48.
[5]	殷丽萍, 刘思远, 蔡成梅, 石小丽, 朱小娟, 袁国明, 汪吉东, 张辉, 徐聪, 马洪波. 嘉博文土壤调理剂对萝卜产量品质、土壤理化性质的研究[J]. 中国农学通报, 2023, 39(6): 64-71.
[6]	王艳, 杨远宁, 黄廉康, 王学礼, 欧昆鹏. 施硒方式对粉葛硒分配及产量和品质的影响[J]. 中国农学通报, 2023, 39(5): 81-86.
[7]	刘爱杰, 张惠忠, 王茂芊. 亚硒酸钠浸种对盐胁迫下甜菜种子萌发的影响[J]. 中国农学通报, 2023, 39(35): 20-26.
[8]	于子惠, 赵成娟, 朱坤淼, 曲昭杰, 胡立勇, 原保忠. 叶面喷施木醋液对萝卜、白菜生长及菌核病抗性的影响[J]. 中国农学通报, 2023, 39(34): 47-52.
[9]	吴悦菊, 张晓杰, 贾卓隆, 周伊鑫, 黄平, 王晓明, 彭建营. 梨果皮色素含量的测定方法研究[J]. 中国农学通报, 2023, 39(28): 119-125.
[10]	王萌, 解晓红, 解红娥, 王凌云, 李江辉, 吴宇浩, 张鸿兴. 桔黄肉早熟鲜食型甘薯新品种‘运薯9801’的选育[J]. 中国农学通报, 2023, 39(27): 24-28.
[11]	刘建香, 郭云周, 杜东英, 张勤斌, 李聪平. 不同有机肥对云南高原红壤生产力的影响[J]. 中国农学通报, 2023, 39(26): 67-75.
[12]	谭嘉川, 胡秀, 钟玉成. 低温弱光对‘寒月’姜花唇瓣花色的影响[J]. 中国农学通报, 2023, 39(25): 42-47.
[13]	韩晓飞, 李潇然, 王俊伟. 沼液替代化肥对露地萝卜产量、品质和土壤理化性质的影响[J]. 中国农学通报, 2023, 39(22): 34-39.
[14]	王亚南, 范雅琦, 霍瑞轩, 姚涛, 郭来春, 葛军勇, 乔月静. 施硒方式对燕麦产量和籽粒硒含量的影响[J]. 中国农学通报, 2023, 39(20): 31-39.
[15]	罗爱国, 冯奕开, 庞琼, 朱敏, 聂萌恩, 杨艳君. 肥料、除草剂和硒配施对谷子品质的影响[J]. 中国农学通报, 2023, 39(18): 11-18.