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中国农学通报

中国农学通报 ›› 2024, Vol. 40 ›› Issue (24): 87-91.doi: 10.11924/j.issn.1000-6850.casb2023-0861

• 资源·环境·生态·土壤 • 上一篇    下一篇

三七籽条对文山红壤土真菌群落结构的影响研究

王灿(), 杨玉玲, 彭翠仙, 赵大伟, 李玲, 杨清松, 孙宏伟, 屈用函, 陶永宏()   

  1. 文山州农业科学院，云南文山 663000
  • 收稿日期:2023-12-07 修回日期:2024-03-20 出版日期:2024-08-20 发布日期:2024-08-20
  • 通讯作者:
    陶永宏，男，1983年出生，云南泸西人，研究员，博士，主要从事三七绿色种植技术研究。通信地址：663000 云南省文山州文山市泰康西路2号 文山州农业科学院，Tel：0876-3037546，E-mail：。
  • 作者简介:

    王灿，男，1993年出生，山西长治人，农艺师，硕士，研究方向：植物中药资源遗传育种。通信地址：663000 云南省文山州文山市泰康西路2号 文山州农业科学院，E-mail：。

  • 基金资助:
    云南省中青年学术和技术带头人后备人才项目(202105AC160003); 以三七为主的中医药产业链关键技术研究专项经费(文财农2023-10); 道地药材（三七、草果）林下生态高效栽培关键技术集成示范项目(202304BP090026); 云南省科技特派队-麻栗坡县下金厂乡铁厂乡科技特派队(WS031020); 文山州兴文英才项目(WS-KJLJ0009)

Effects of Panax notoginseng Seed on Fungal Community Structure in Wenshan Red Loam Soil

WANG Can(), YANG Yuling, PENG Cuixian, ZHAO Dawei, LI Ling, YANG Qingsong, SUN Hongwei, QU Yonghan, TAO Yonghong()   

  1. Wenshan Academy of Agricultural Sciences, Wenshan, Yunnan 663000
  • Received:2023-12-07 Revised:2024-03-20 Published:2024-08-20 Online:2024-08-20

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摘要：

植物早期生长对根际土壤微生物群落有显著调控作用。本研究旨在探讨三七籽条根际土壤与非根际土壤中真菌群落结构的差异。通过采集健康三七籽条根际土壤（HA）和附近未种植三七籽条的非根际土壤（CK），利用ITS rRNA高通量测序技术进行分析。结果表明，非根际土壤中真菌群落目、科、属、种个数显著高于(P<0.05)三七籽条根际土壤，其中门分类水平下，真菌群落大致由子囊菌门Ascomycota (CK 50.84%，HA 78.12%)、k__Fungi_Unclassified (CK 17.96%，HA 5.09%)、担子菌门Basidiomycota (CK 16.50%，HA 2.53%)组成。多样性分析中非根际土壤ace、chao1和Shannon、Simpson均显著高于根际土壤(P<0.05)。PCoA分析显示根际与非根际土壤真菌群落结构有显著差异，群落相似性较低(R2=0.6776，P=0.005)。富集分析显示非根际土壤中有较多真菌群类富集，如担子菌门Basidiomycota、座囊菌纲Dothideomycetes、散囊菌纲Eurotiomycetes等。在根际土壤中真菌类群则表现的较为单一，仅在子囊菌门Ascomycota、粪壳菌纲Sordariomycetes、炭角菌目Xylariales中显著富集，如木霉属Trichoderma、帚霉属Scopulariopsis、明梭孢属Monographella。其中木霉属Trichoderma (CK 0.33%，HA 1.73%)是有具有生物防控功能的微生物，可促进植物生长。

关键词: 三七籽条, 真菌, 群落多样性, ITS rRNA, 红壤土, 真菌群落结构, 高通量测序, 生物防控, 植物生长

Abstract:

The early development of plants plays a crucial role in shaping the microbial community within the rhizosphere soil. This study aims to investigate the impact of Panax notoginseng seedling strips on the fungal community structure in red loam soil. Rhizosphere soil (HA) samples were collected from healthy P. notoginseng seedling strips, while non-rhizosphere soil (CK) samples were obtained without any P. notoginseng seedlings. High-throughput sequencing was performed on these samples using ITS rRNA. The results showed that the number of orders, families, genera and species in non-rhizosphere soil was significantly higher than those in the rhizosphere soil of P. notoginseng seedlings (P<0.05). At the taxonomic level, Ascomycota accounted for a major proportion of fungal communities (CK 50.84%, HA 78.12%), followed by k__Fungi_Unclassified (CK 17.96%, HA 5.09) and Basidiomycota (CK 16.50%，HA 2.53%). Diversity analysis revealed significantly higher values for ace, chao1, Shannon and Simpson indices in non-rhizosphere soil compared to rhizosphere soil (P<0.05). PCoA analysis demonstrated a significant difference in fungal community structure between rhizosphere and non-rhizosphere soils with low community similarity (R2=0.6776，P=0.005). Enrichment analysis identified several fungi groups enriched in non-rhizosphere soil including Basidiomycota, Dothideomycetes, and Eurotiomycetes; whereas only Ascomycota, Sordariomycetes and Xylariales were significantly enriched in rhizosphere soil including Trichoderma, Scopulariopsis and Monographella. Notably, Trichoderma (CK 0.33%，HA 1.73%) was known as a biocontrol microorganism capable of promoting plant growth.

Key words: P. notoginseng seedling, fgungal community, community diversity, ITS rRNA, red loam, fungal community structure, high-throughput sequencing, biological control, plant growth