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中国农学通报

中国农学通报 ›› 2024, Vol. 40 ›› Issue (36): 51-56.doi: 10.11924/j.issn.1000-6850.casb2023-0446

• 林学·园艺·园林 • 上一篇    下一篇

木质素沉积对种皮开裂的影响

李昕然(), 刘畅, 冯国军, 刘大军, 安普南, 杨晓旭   

  1. 黑龙江大学现代农业与生态环境学院，哈尔滨 150000
  • 收稿日期:2023-06-23 修回日期:2023-09-15 出版日期:2024-12-25 发布日期:2024-12-23
  • 通讯作者:
    杨晓旭，男，1988年出生，吉林松原人，副研究员，博士，研究方向：蔬菜遗传育种与生物技术。通信地址：150000 黑龙江省哈尔滨市南岗区学府路74号黑龙江大学农学楼，Tel：15546226629。
  • 作者简介:

    李昕然，女，2000年出生，黑龙江哈尔滨人，硕士研究生，研究方向：农业资源保护与利用。通信地址：150000 黑龙江省哈尔滨市南岗区学府路74号黑龙江大学农学楼，Tel：13936052989，E-mail：。

  • 基金资助:
    黑龙江省重点研发计划项目专项课题计划“主要蔬菜作物分子辅助育种决策与新品系培育研究”(SC2022ZX02C0202); 农业农村部政府购买合同“普通菜豆种质资源品质性状鉴定”(19240656); 2022年度省属高校基本科研业务费项目(2022-KYYWF-1071)

Effect of Lignin Deposition on Seed Coat Cracking

LI Xinran(), LIU Chang, FENG Guojun, LIU Dajun, AN Punan, YANG Xiaoxu   

  1. College of Modern Agriculture and Ecological Environment, Heilongjiang University, Harbin 150000
  • Received:2023-06-23 Revised:2023-09-15 Published:2024-12-25 Online:2024-12-23

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摘要：

自然条件下收获的菜豆种子经常会出现种皮开裂的现象，为了探讨菜豆种子种皮开裂的原因，改善种子的外观表现与活力，本研究以黑大黛冠、黑大盛冠为实验材料，将同品种的正常种子与种皮开裂(SC)种子进行对照，对其长、宽、厚、百粒重及物理性能进行测定，并在生化和分子水平上研究了低温对苯丙烷代谢途径的影响。结果表明：开裂种子长、宽、厚分别大于正常种子11.14%、2.79%、10.15%，百粒重显著提高16.79%，物理性能降低，苯丙烷代谢途径在转录水平上增强。推测开裂种子的子叶发育速率大于正常种子，子叶与种皮发育速率不统一可能是导致种皮开裂的原因之一；低温使得种皮内木质素含量上升，木质素沉积使得种皮物理性能降低，是导致种皮开裂的直接原因。

关键词: 菜豆, 种皮开裂, 低温, 苯丙烷代谢途径, 木质素, 百粒重

Abstract:

Seed coat cracking often occurs in bean seeds harvested under natural conditions. In order to explore the causes of seed coat cracking and improve seed appearance and vigor, we chose the Heilongjiang University ‘Daiguan’and the Heilongjiang University‘Shengguan’as experimental materials, and compared between intact and seed coat cracking (SC) seeds of the same variety. Their length, width, thickness, 100-seed weight and physical properties were measured, and the effect of Phenylpropane metabolic pathway in low temperature was studied at biochemical and molecular level. The results showed that the length, width and thickness of cracked seeds were 11.14% 2.79% 10.15% larger than that of intact seeds respectively, the 100-grain weight increased significantly by 16.79%, the physical properties decreased, and phenylpropane metabolic pathway was enhanced at the transcriptional level. It is speculated that the cotyledon development rate of cracked seeds is higher than that of intact seeds, and the difference of cotyledon and seed coat development rate may be one of the reasons leading to seed coat cracking; low temperature increases the content of Lignin in the seed coat, and the deposition of Lignin lowers the physical properties of the seed coat, which is the direct cause of seed coat cracking.

Key words: common bean, seed coat cracking, low temperature, phenylpropane metabolic pathway, lignin, hundred-grain weight