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中国农学通报

中国农学通报 ›› 2007, Vol. 23 ›› Issue (1): 6-006.

所属专题： 生物技术； 畜牧兽医

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奶牛乳房炎抗性相关基因Znf313的电子克隆与序列分析

曹随忠,,,李宏滨,姚学萍,赵兴绪,杜立新   

  • 出版日期:2007-01-05 发布日期:2007-01-05

In Silico Cloning and Characterization of Mastitis Resistant Gene Znf313 from Dairy Cows

Cao Suizhong, , , Li Hongbin, Yao Xueping, Zhao Xingxu, Du Lixin   

  • Online:2007-01-05 Published:2007-01-05

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摘要： 电子克隆是基因克隆的新策略。利用一个患乳房炎奶牛外周血白细胞中差异表达的EST序列为信息探针进行电子克隆，根据电子克隆序列设计引物，以奶牛外周血白细胞cDNA为模板进行RT-PCR验证，PCR产物克隆入pGEM-T质粒载体，测序得到的序列已被GenBank收录（DQ010409）。该基因被命名为牛锌指蛋白313(Znf313)基因，开放阅读框1~693bp，推测编码230个氨基酸，预测其编码蛋白分子量为25.67kD，等电点6.90。

关键词: 姜瘟病, 姜瘟病, 生物防治, 拮抗细菌, 抑菌圈

Abstract: In silico cloning was a new strategy of gene cloning developed with the development of genome, EST projects and bio-informatics. Using one differentially expressed EST in peripheral blood leukocytes of Holstein cows with mastitis as a querying probe, a novel cDNA sequence was obtained from bovine EST database of GenBank, which was named bovine zinc finger protein 313(Znf313) gene. The sequence of in silico cloning was verified by RT-PCR, and inserted into pGEM-T vector for sequencing. The bovine Znf313 gene cDNA contains a 693bp open reading frame, encoding 230aa protein, putative protein molecular weight is 25.67kD, isoelectric point is 6.90. The sequence has been submitted to GenBank with the accession number DQ010409.

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