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中国农学通报

中国农学通报 ›› 2007, Vol. 23 ›› Issue (9): 8-8.

所属专题： 生物技术； 畜牧兽医

• 畜牧 动物医学 蚕 蜂 • 上一篇    下一篇

绵羊intelectin基因cDNA克隆与序列分析

杨 森,杨国宇,郭豫杰   

  • 出版日期:2007-09-05 发布日期:2007-09-05

Molecular Cloning and Sequence Analysis of Sheep Intelectin cDNA

Yang Sen, Yang Guoyu, Guo Yujie   

  • Online:2007-09-05 Published:2007-09-05

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摘要： 【研究目的】克隆并分析绵羊intelectin基因；【方法】十二指肠粘膜组织提取总RNA，分别以上游电子克隆的拼接体和下游保守区为模板设计引物进行RT-PCR，PCR产物与pMD-19T载体连接后转化E. coli JM109，筛选阳性克隆并测序；【结果】克隆的绵羊intelectin基因与牛、猪、人、大鼠的同源性分别为93%、87%、83%、79% ，预测的氨基酸序列包含FBG结构域。【结论】成功克隆了绵羊intelectin-2基因，并注册GenBank (Accession. EF624459)

关键词: 茶饮料, 茶饮料, 沉淀, 澄清技术, 应用

Abstract: 【Objective】To clone and analysis the sheep intelectin . 【Method】Total RNA was extracted from duodenal mucosa tissue and mRNA sequence of gene were amplified by RT-PCR using designed primers, the upstream of which was designed from the contig by silico cloning, the downstream of which was designed in conservative region. The PCR products were ligated into the pMD-19T vector, and then transformed into competent cells of E. coli JM109.The sequence was analyzed to identify the recombinant plasmid. 【Results】Identity analysis showed that the intelectin nucleotide sequence shared 93.3%, 87.1% ,82.9% and 78.6% homology with that of bovine (predicted), porcine , human and rat. The predicted peptide contained a FBG motif. 【Conclusion】 The sheep intelectin-2 was successfully cloned and the sequence has been submitted to GenBank(Accession. EF624459).

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