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中国农学通报

中国农学通报 ›› 2007, Vol. 23 ›› Issue (12): 32-32.

所属专题： 生物技术

• 目次 • 上一篇    下一篇

鹅生长激素受体基因荧光定量PCR检测方法的建立

杨 隽,张 才,肖翠红,李 馨   

  • 出版日期:2007-12-05 发布日期:2007-12-05

Development of Real-time PCR Assay for Geese Growth Hormone Receptor Gene

Yang Jun, Zhang Cai, Xiao Cuihong, Li Xin   

  • Online:2007-12-05 Published:2007-12-05

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摘要： 根据GenBank中鹅生长激素受体（GHR）基因序列设计合成了引物和探针，对荧光定量PCR的方法进行了方法学的评估，建立了TaqmanMGB荧光定量RT-PCR检测方法。结果表明，由pMD-18T-GHR所构建的标准曲线线性关系良好，建立的GHR基因荧光定量PCR检测方法灵敏度高、特异性强（可以检测出低于10个拷贝/μl的样品），准确可靠。籽鹅和莱茵鹅GHR mRNA出壳到90日龄生长过程中肝脏中的表达量不同，30日龄不同组织的表达量各有变化特点。

关键词: 聚类分析, 聚类分析, 配方, 相似性

Abstract: The probe and primers were designed and synthesized according to the growth hormone receptor sequence of goose available in GenBank. Then a real-time RT-PCR assay was developed and assessed. Results showed that the standard curve made by pMD-18T-GHR had good linear dependence, and the fluorescent quantitative RT-PCR assay was sensitive, specific and reliable. The expression of GHR mRNA were difference in liver during postnatal 90 days , and there was different changing pattern in other tissues at 30 days between Zigeese and Rhine.

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