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中国农学通报

中国农学通报 ›› 2009, Vol. 25 ›› Issue (4): 149-156.

所属专题： 生物技术； 食用菌； 食用菌

• 林学 园艺 园林 • 上一篇    下一篇

双孢蘑菇栽培菌株遗传多样性的DNA指纹分析

陈美元,廖剑华,李洪荣,等   

  • 收稿日期:2008-10-30 修回日期:2009-01-07 出版日期:2009-02-20 发布日期:2009-02-20

DNA fingerprinting of genetic diversity of Agaricus bisporus cultivation strains

  • Received:2008-10-30 Revised:2009-01-07 Online:2009-02-20 Published:2009-02-20

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摘要： 对双孢蘑菇206个栽培菌株的总DNA进行大规模的SRAP、ISSR和RAPD分析，共获得15条SRAP、3条ISSR和2条RAPD标记。利用这20个标记对206个菌株进行聚类分析，获得亲缘关系树状图。结果显示在28%的相似值上，206个菌株可以分为高产型和优质型两大类群，而在100%相似值上，可分为大小72个类群，每群包含菌株数为1~31不等。

关键词: 动态变化, 动态变化, 转换系数, 耕地重心, 驱动机制, 皖江地区

Abstract: 206 cultivation strains of Agaricus bisporus were studied by using the techniques of SRAP, ISSR and RAPD fingerprinting, and 15 special SRAP bands, 3 special ISSR bands and 2 special RAPD bands were obtained. The strains were clustered based on the 20 special bands and a tree map was obtained. The results showed that 206 strains be divided into high-production group and good-quality group at the similarity coefficient of 28%, while at the similarity coefficient of 100%, the strains were divided into 72 groups with strain number range of 1~31.

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