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中国农学通报 ›› 2009, Vol. 25 ›› Issue (4): 205-207.

所属专题: 生物技术 园艺

• 林学 园艺 园林 • 上一篇    下一篇

聚乙烯醇在草莓苗组织培养中的作用

刘雪静,代 丽,尹宝重,等   

  • 收稿日期:2008-11-27 修回日期:2008-12-14 出版日期:2009-02-20 发布日期:2009-02-20

Effects of Polyvinyl alcohol on tissue culture of Strawberry

  • Received:2008-11-27 Revised:2008-12-14 Online:2009-02-20 Published:2009-02-20

摘要: 以丰香草莓组培苗为试材,研究了不同浓度聚乙烯醇对草莓组培苗叶片愈伤组织诱导、继代增殖及生根培养的影响。结果表明,1g/L的聚乙烯醇可使草莓组培苗叶片愈伤组织发生期提前,提高愈伤组织发生率和发生量,改变愈伤组织的质量,同时能有效降低草莓组培苗在继代增殖中的玻璃化现象,促进植株分化、提高叶绿素和蛋白的含量。2g/L的聚乙烯醇可以提高组培苗在生根培养基中的生根条数、根长度、株高、叶绿素和蛋白含量等。

关键词: 宁夏, 宁夏, 农村环境, 现状, 保护对策

Abstract: The tissue culture of Fengxiang strawberry was used as experimental materials. The effects of PVA on tissue culture of strawberry were studied. The results indicated that 1g/L PVA can reduce the rate of vitrified plantlets of strawberry significantly during proliferating, and can also improve the contenes of chlorophyll and albumen. During leaf culture, 1g/L PVA may quicken callus emergence and increase the rate of callus formation and improve the quality of callus. 2g/L PVA can improve the root number of single plantlet, the length of roots, the high of plantlet, the contents of chlorophyll and albumen.

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