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中国农学通报

中国农学通报 ›› 2009, Vol. 25 ›› Issue (5): 38-42.

所属专题： 生物技术

• 生物技术科学 • 上一篇    下一篇

桃花粉管通道法转基因技术的初步研究

万春雁,韩明玉,赵彩平,侯玉珏,丛 芳,田玉命   

  • 收稿日期:2008-11-27 修回日期:2008-12-24 出版日期:2009-03-05 发布日期:2009-03-05

A Preliminary Study On Transgenic Technology Of Pollen-tube PathWay In Peach (Prunus persical.Batsch)

Wan Chunyan, Han Mingyu, Zhao Caiping, Hou Yujue, Cong Fang, Tian Yuming   

  • Received:2008-11-27 Revised:2008-12-24 Online:2009-03-05 Published:2009-03-05

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摘要： 以曙光为试材，对桃花粉管通道法转基因技术进行探索性研究，结果表明：（1）活体人工授粉后，24h花粉开始在柱头上萌发授粉后48h已有大量的花粉管长至花柱上端；授粉后96h大量的花粉管生长至花柱的中部；授粉后144h大量的花粉管到达花柱基部。（2）确定外源DNA的注射时间为人工授粉后的144h；花柱横切滴加法注射方式不适用于桃；当外源质粒DNA浓度为0.2 mg/l时，发育期内其坐果率都显著高于其它浓度水平。

关键词: 土样拮抗菌, 土样拮抗菌, 放线菌分离, 抑制剂, 生物防治

Abstract: Experiment was conducted with Shuguang(Prunus persical.Batsch) for studying the applying of Pollen-tube path way in peach.Results showed: (1)24h after pollination in vivo, pollens germinated on the stigma;After 48h ,96h,144h plenty of pollen-tubes respectively reached to the upper,middle,bottom of style.(2) The best time to inoculate was 144h after pollination; The injection method of adding the crosscut on the stigma was not suitable for peach;The rate of fruit setting was better than other concentration when was 0.2 mg/l.