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中国农学通报

中国农学通报 ›› 2009, Vol. 25 ›› Issue (16): 39-44.

所属专题： 生物技术； 园艺

• 生物技术科学 • 上一篇    下一篇

相思树的愈伤组织培养及其组织细胞学观察

叶玲娟,赖钟雄,苏齐珍,林姗姗,林庆良   

  • 收稿日期:2008-12-26 修回日期:2009-03-06 出版日期:2009-08-20 发布日期:2009-08-20

The callus culture and cytohistological observation on Acacia. spp

Ye Lingjuan, Lai Zhongxiong, Su Qizhen, Lin Shanshan, Lin Qingliang   

  • Received:2008-12-26 Revised:2009-03-06 Online:2009-08-20 Published:2009-08-20

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摘要： 本文研究了相思愈伤组织继代培养过程中，激素浓度、光照条件、接种量等各因素对相思愈伤组织培养的影响，研究结果发现，在培养基中添加0.2～0.5mg/L的2，4-D浓度水平最有利于相思愈伤组织的生长。通过不同光照强度的对比试验得出，弱光在保持愈伤组织的形态上较有利。将接种量控制在0.3 g/瓶时，在愈伤组织的增殖和保持能达到最优的效果。同时，通过对不同相思愈伤组织细胞学观察可以看出，不同相思品种的愈伤组织细胞形态各不相同，在培养过程中，愈伤组织的细胞的形态也在变化。

关键词: 节节麦, 节节麦, PEG6000, 水杨酸, 活性氧, 抗氧化酶

Abstract: The different effects such as plant growth regulators, light conditions, and genotypes on the maintenance of the three species of Acacia were compared in the experiment, and the results showed that the medium supplemented with 0.2-1 mg昄-12,4-D was beneficial to the maintenance of calli. Moreover, the calli could be kept loose and soft under the weak light by comparing the effects of different light conditions. And the superior inoculation amount was 0.3 g calli in each bottle. By the cytohistological observation on different calli in acacia, it showed that there were different Morphological cells and calli in different species of Acacia, which also changed during the culture.