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中国农学通报

中国农学通报 ›› 2009, Vol. 25 ›› Issue (16): 32-38.

所属专题： 棉花

• 生物技术科学 • 上一篇    下一篇

利用SRAP标记分析彩色棉与白色棉的遗传差异

凌 磊,李廷春,李正鹏,蔡 沂,孙 旭,苏 翔,林 毅,蔡永萍   

  • 收稿日期:2009-04-09 修回日期:2009-04-17 出版日期:2009-08-20 发布日期:2009-08-20

Genetic Difference Analysis between Colored Cotton and White Cotton by SRAP Markers

Ling Lei, Li Tingchun, Li Zhengpeng, Cai Yi, Sun Xu, Su Xiang, Lin Yi, Cai Yongping   

  • Received:2009-04-09 Revised:2009-04-17 Online:2009-08-20 Published:2009-08-20

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摘要： 利用SRAP分子标记技术对彩色棉遗传多样性进行研究，应用NTSYS软件对30种供试棉花材料的SRAP-PCR结果进行分析，从中筛选得到29对条带清晰的多态性引物，共产生1067条清晰条带，多态性条带132条，平均每个引物组合得到4.55条多态性条带。聚类分析29对引物组合的扩增结果，Jaccard’s相似系数在0.5405-0.9109之间，利用SRAP标记可以判明彩色棉和白色棉的遗传差异，可有效地应用于彩色棉的遗传多样性及亲缘关系的研究。

关键词: 麋鹿, 麋鹿, 狼尾草, 生物量, 生物多样性, 大丰麋鹿自然保护区

Abstract: The genetic diversity of colored cotton was studied by SRAP, and the results of SRAP-PCR of 30 spices of cotton materials were analyzed by NTSYS software. Twenty-nine primer combinations were selected from 88 primer pairs , which amplified 132 polymorphic bands with an average of 4.55 polymorphic bands per primer pair. Cluster analysis by using UPGMA method based on the data of SRAP amplified bands by 29 primer pairs showed that Jaccard’s similarity coefficient ranged from 0.5405 to 0.9109. The results indicated that the genetic difference between colored cotton and white cotton can be distinguished by SRAP, and SRAP molecular marker can be applied to study the colored cotton genetic diversity efficiently.