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中国农学通报

中国农学通报 ›› 2009, Vol. 25 ›› Issue (16): 28-31.

所属专题： 畜牧兽医

• 畜牧 动物医学 蚕 蜂 • 上一篇    下一篇

一种新的鸭病（暂名鸭出血性坏死性肝炎）病原学研究初报

陈少莺,陈仕龙,林锋强,江斌,王劭,程晓霞,朱小丽,张世忠,李兆龙,程由铨   

  • 收稿日期:2009-05-05 修回日期:2009-05-20 出版日期:2009-08-20 发布日期:2009-08-20

The Primary Study of Pathogen of Duck Hemorrhagic-necrotic hepatitis

Chen Shaoying,, Chen Shilong,, Lin Fengqiang,, Jiang Bin,, Wang Shao,, Cheng Xiaoxia,, Zhu Xiaoli,, Zhang Shizhong,, Li Zhaolong,, Cheng Youquan   

  • Received:2009-05-05 Revised:2009-05-20 Online:2009-08-20 Published:2009-08-20

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618

摘要： 从临床表现为肝脏不同程度点/斑块状出血和坏死为主要病变的病死雏番鸭、雏半番鸭和雏麻鸭肝脾组织中分离到8株病毒。分离毒能致死番鸭胚和鸡胚，胚体充出血、胚肝肿大出血坏死；人工感染1-2日龄雏番鸭、雏半番鸭均能复制出与临床自然发病鸭相同的临床症状和病理变化，并能回收到病毒；分离毒能在MDEF中增殖并产生细胞病变，经电镜观察，病毒在细胞浆中增殖，呈大量散在、成堆和晶格状排列，病毒粒子呈球形、二十面体对称、无囊膜、双层衣壳、直径70nm左右；分离毒不能凝集鸡和鸽红细胞，对乙醚、氯仿、FUDR不敏感；病毒核酸为dsRNA，在SDS-PAGE中具有禽呼肠孤病毒10个RNA片段的特征(L1-3、M1-3 和 S1-4)；应用MDRV特异性引物不能从分离毒中扩增出任何条带；鉴于分离毒的上述特性，暂将此分离毒归属于呼肠孤病毒科正呼肠孤病毒属新型鸭呼肠孤病毒。

关键词: 椰子, 椰子, 组织培养, 胚培养, 未成熟花序

Abstract: Eight virus strains were isolated from the liver and spleen of the dead young muscovy ducks, mule duck and sheldrake characterized with symptoms of different degree dot/spot haemorrhage and necrosis in the liver. These isolates could caused the muscovy duck- embryo and chick-embryo to die that showed the hemorrhage and necrosis in embryo-livers. 1-2 old-day birds above infected with these isolates had the same character with clinically dead birds and the virus could be isolated from artificial infected birds. These isolates could proliferate in MDEF and result in CPE.The virus could proliferate in the cytoplasm in order of crystals. The viron was shown spherical, icosahedron, cubic symmetry, no-envelope,with double-layered capsid, about 70nm in diameter by electron microscopy. These isolates had no haemagglutination activity and were not sensitive with chloroform, ether and FUDR. The PAGE analysis of the viral genome revealed that isolates genome were related to avian reovirus (ARV), composed of ten segments of dsRNA (L1-3、M1-3 and S1-4). Moreover, the specific band could not be amplified from isolates by specific primers of MDRV. Above all, these isolates temporarily belong to new-type duck reovirus, Orthoreovirus Reoviridae