油柿叶片离体再生体系的建立

中国农学通报 ›› 2008, Vol. 24 ›› Issue (8): 136-139.

油柿叶片离体再生体系的建立

谢启鑫, ,庄东红,

收稿日期:2008-05-28 修回日期:2008-06-11 出版日期:2008-08-08 发布日期:2008-08-08

Developing of Plant Regeneration System from Leaf Explants of Date Plum (Diospyros oleifera Cheng)

Xie Qixin, , Zhuang Donghong,

Received:2008-05-28 Revised:2008-06-11 Online:2008-08-08 Published:2008-08-08

摘要/Abstract

摘要： 以油柿叶片为材料开展了离体培养研究，旨在建立其再生体系，为转基因操作奠定基础。结果表明，油柿叶盘在MS（1/2N）+IBA0.1mg/L+ ZT3.0mg/L培养基上，经前期暗处理3周后移至正常光照下培养的再生效果最好，其不定芽再生率和外植体平均不定芽数最高，分别为80.5%和（4.1±0.8）个。再生苗接种于添加IAA和IBA各0.5mg/L的MS(1/2N)培养基上，4周时生根率可达92%，成功地建立了油柿叶片的离体再生体系。

关键词: 荒漠化, 荒漠化, 农村, 能源利用

Abstract: In order to developing a plant regeneration system of Diospyros oleifera Cheng thereby establish a foundation for transformation, shoot regeneration and rooting from leaf explants were studied. The result showed that modified Murashige and Skoog (MS)[MS(1/2N)] containing 0.1 mg/L indole butyric acidand 3.0 mg/L zeatin was the most optimum for shoot regeneration when a 3 weeks previous dark treatment was added. The shoot percentage and average shoots number per explant were 80.5% and (4.1±0.8) respectively. As for regenerated shoots rooting, the modified Murashige and Skoog containing 0.5 mg/L both indole acetic acid and indole butyric acid was most effective, in which rooting percentage reached up to 92% after 4 weeks. The plant regeneration system of Diospyros oleifera Cheng has been developed successfully.

中图分类号:

S665

谢启鑫, ,庄东红,. 油柿叶片离体再生体系的建立[J]. 中国农学通报, 2008, 24(8): 136-139.

Xie Qixin, , Zhuang Donghong,. Developing of Plant Regeneration System from Leaf Explants of Date Plum (Diospyros oleifera Cheng)[J]. Chinese Agricultural Science Bulletin, 2008, 24(8): 136-139.

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