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中国农学通报

中国农学通报 ›› 2008, Vol. 24 ›› Issue (8): 131-135.

所属专题： 生物技术

• 生物技术科学 • 上一篇    下一篇

大叶黄杨幼茎愈伤组织诱导的研究初报

曹 彬,孙占育,张德仓   

  • 收稿日期:2008-04-25 修回日期:2008-05-31 出版日期:2008-08-08 发布日期:2008-08-08

Preliminary Study on Callus Induction of Tender Stem of Euonymus Japonicus

Cao Bin, Sun Zhanyu , Zhang Decang   

  • Received:2008-04-25 Revised:2008-05-31 Online:2008-08-08 Published:2008-08-08

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摘要： 以大叶黄杨的幼茎段为外植体，在添加BA和NAA、IBA、2,4-D不同激素组合的MS培养基上培养，对其愈伤组织进行诱导试验。结果表明：①生长素对愈伤组织诱导的效应为2,4-D >NAA>IBA；②在不同激素组合培养基上均可诱导出愈伤组织，其中MS+BA1.0~2.0 mg/L+NAA 1.0~1.5 mg/L、MS+BA1.5~2.0 mg/L+IBA1.0~1.5 mg/L、MS+BA0.5~1.0 mg/L+2,4-D 0.5~1.5 mg/L等对愈伤组织的诱导效果最好，其诱导率分别为74.3%、65.3%、81.1%。因此,通过科学配制不同激素组合的MS培养基，就能有效地诱导出大叶黄杨幼茎的愈伤组织。

关键词: 过碳酰胺, 过碳酰胺, 土壤, pH, 氨气挥发, 过碳酰胺N转化, 活性铝

Abstract: With young stem segment of Euonymus japonicus as explants, callus induction were studied on MS basal medium supplemented with different hormone combinations including BA and NAA、IBA 、2,4-D. The result indicated that (1) The effect sequence of auxin was 2,4-D > NAA >IBA from the callus induction. (2) Callus were induced on medium of different hormone combinations，and MS+BA1.0~2.0 mg/L+NAA 0.5~1.5 mg/L、 MS+BA1.5~2.0 mg/L+ IBA1.0~1.5mg/L、 MS+BA0.5~1.0 mg/L+2,4-D 0.5~1.5mg/L etc. was the best one for callus induction，and the rate of induction was respectively 74.3%、65.3%、81.1%. So through scientific preparation，callus induction of young stem can be induced effectively，on MS medium with different hormone combinations.

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