关键词: 攀枝花苏铁, 攀枝花苏铁, ISSR, 影响因子, PCR, 反应体系

Abstract: Abstract: [Objective] Understand the type and characteristic of the Root-Knot Nematode in the clerodendranthus spicatus.(Thunb.)C.Y.Wu and the influence which grows to the clerodendranthus spicatus.[Method] Separates the Root-Knot Nematode’s female and male and larva from root system which the clerodendranthus spicatus. were infected with the Root-Knot Nematode, view and describe the Root-Knot Nematode’s characteristic under a microscope and discern the type through compares the Root-Knot Nematode’s characteristic pattern. Study the influence of growth which the clerodendranthus spicatus. were infected through the pathogenic experiment, and make an objective appraisa through viewing the clerodendranthus spicatus.’s growing trend and setting-out content of the chlorophyll and protein.[Result] Separated and get the clerodendranthus spicatus. (Thunb.)C.Y.Wu.’s Root-Knot Nematode, and has succeeded in doing the pathogenic experiment .[Conclusion] The Root-Knot Nematode in the clerodendranthus spicatus.(Thunb.)C.Y.Wu is the Meloidyne incognita. .It is serious interfere with the clerodendranthus spicatus.’s growth. The clerodendranthus spicatus. is doing bad even died, the important metabolism material like protein and chlorophyll’s content is obvious drops.