野生香蕉叶片总RNA提取方法研究

中国农学通报 ›› 2009, Vol. 25 ›› Issue (14): 51-54.

野生香蕉叶片总RNA提取方法研究

张妙霞,赖钟雄

收稿日期:2009-02-27 修回日期:2009-04-17 出版日期:2009-07-20 发布日期:2009-07-20

The Method of Total RNA Isolation from the Wild Banana Leaves

ZHANG Miao-Xia ,

Received:2009-02-27 Revised:2009-04-17 Online:2009-07-20 Published:2009-07-20

摘要/Abstract

摘要： 采用TRIZOL试剂盒法、RNAsio试剂法和改良CTAB法提取香蕉叶片总RNA，并通过凝胶电泳、紫外分光光度法检测提取的RNA样品的质量。研究结果表明：改良CTAB法提取的RNA具有28SrRNA和18SrRNA两条清晰的条带，OD260／OD280在1.80-1.95之间，OD260／OD230在1.75-2.10之间，具有较高的纯度；其它两种方法获得的RNA纯度不够，降解严重。将提取的RNA分别逆转录成cDNA，经RT-PCR扩增，只有改良CTAB法出现清晰的条带，进一步证明改良CTAB法提取的RNA具有很高的纯度，可以满足进一步分子生物学研究的要求。

关键词: 迷迭香, 迷迭香, 不同基质, 扦插, 育苗

Abstract: Total RNA was isolated from wild banana leaves by using the methods of TRIZOL Regent kit，RNAsio Regent and improved CTAB. The quality of total RNA was analyzed through gel electrophoresis and UV spectrometer. The results indicated that the RNA isolated by the method of improved CTAB showed clear bands of 28SrRNA and 18SrRNA，and the value of OD260／OD280 was 1.80-1.95，the value of OD260／OD230 was 1.75-2.10. RNA isolated by the other two methods degraded seriously．RNA could be reverse to cDNA．The cDNA was used for RT-PCR amplifying，but the electrophoresis figure by RT-PCR showed that there was one distinct band using the RNA templet isolated by the method of CTAB. These results demonstrated that the quality and purity of the RNA obtained by the method of improved CTAB could meet the demands of molecular biology experiment．

张妙霞,赖钟雄. 野生香蕉叶片总RNA提取方法研究[J]. 中国农学通报, 2009, 25(14): 51-54.

ZHANG Miao-Xia ,. The Method of Total RNA Isolation from the Wild Banana Leaves[J]. Chinese Agricultural Science Bulletin, 2009, 25(14): 51-54.

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