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中国农学通报

中国农学通报 ›› 2009, Vol. 25 ›› Issue (9): 11-18.

• 生物技术科学 • 上一篇    下一篇

利用陆海杂种BC1群体构建遗传连锁图谱并初步定位产量性状相关的QTL

李骏智,,杨泽茂,,李俊文,石玉真,刘爱英,陈 琴,, 李爱国,张保才,刘广平,蒋建雄,王 涛,袁有禄   

  • 收稿日期:2009-03-20 修回日期:2009-04-08 出版日期:2009-05-05 发布日期:2009-05-05

Using G. hirsutum×G. barbadense BC1 Populations Construction Genetic Linkage Map and Primary QTL Analysis of Yield Related Traits

Li Junzhi,, Yang Zemao,, Li Junwen, Shi Yuzhen, Liu Aiying, Chen Qin,, Li Aiguo, Zhang Baocai, Liu Guangping, Jiang Jianxiong, Wang Tao, Yuan Youlu   

  • Received:2009-03-20 Revised:2009-04-08 Online:2009-05-05 Published:2009-05-05

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摘要： 摘要：本研究利用中棉所36和海1配制杂交组合，并用中棉所36为轮回亲本构建回交群体(BC1F1, BC2F1和 BC1S1)。用亲本和F1对新开发的2102对SSR引物进行多态性筛选，共筛选到317对含有海1显性带的引物，占筛选引物总数的15.08%；最终对其中的275对引物进行了BC1F1群体扩增，获得306个SSR标记差异位点。连锁分析表明（LOD=6.5），有254个标记位点连锁，分布在42个连锁群中，覆盖2252.36cM，约占棉花基因组的50.05%；平均每个连锁群有6.08个标记，覆盖53.63 cM；标记间平均间距为8.87cM。利用BC1F1、BC2F1和BC1S1三个不同世代分离群体产量性状数据，共定位16个产量性状QTL，解释表型变异5.77%～19.86%。其中，衣分6个，铃重6个，籽指4个。有9个增效基因来自陆地棉亲本中棉所36，7个增效基因来自海岛棉亲本海1，说明了表型性状较差的品种同样可能含有可用于性状改良的增效基因。控制衣分的3个QTL可在不同的世代稳定检测到，效应稳定，增效基因均来自高值亲本陆地棉，为进一步分子标记辅助选择奠定了基础。

关键词: 加工番茄植株, 加工番茄植株, 连作障碍, 根系活力, 膜透性, SOD, CAT, POD, MDA

Abstract: Abstract: The G. hirsutum ZMS36 and the G. barbadense Hai1 were crossed to construct backcross populations (BC1F1, BC2F1, and BC1S1) with ZMS36 as recurrent parent. A total of 2102 pairs of SSR primer were used to screen polymorphism among the parents ZMS36, Hai 1 and their F1, which resulted in 317 pairs of polymorphic primer with the special band for Hai 1, and the polymorphism primer accounted for 15.08% of the total primer, and 275 of them were used to screen the BC1F1 population. Linkage test（LOD=6.5）indicated 254 of 306 polymorphic locus derived from 275 pairs of primer could be mapped into 42 linkage groups and covered a total genetic distance of 2252.36cM, approximately 50.05% of cotton genome. The number of the marker locus per linkage group was 6.08, which spanned 53.63cM of the cotton genome. And the average distance of the neighboring marker locus were 8.87cM. 16 QTLs for yield-related traits were detected from the three generations of BC1F1, BC2F1, and BC1S1 using CIM. QTL numbers for lint percentage, boll weight and seed index were 6, 6 and 4, respectively, which accounted for 5.77%～19.86% of phenotypic variance. Among the 16 QTLs, 9 with positive additive effect derived from ZMS36 and 7 QTLs with negative additive effect from Hai1, which indicates the materials with poor phenotypic characteristics may contain the useful gene for improving the same traits. 3 QTLs for lint percentage could be stably detected in the different generations, and from high-value parent of ZMS36, which should be useful for the marker-assisted selection.