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中国农学通报

中国农学通报 ›› 2009, Vol. 25 ›› Issue (21): 277-280.

• 植物保护 农药 • 上一篇    下一篇

百里香酚抑菌活性初探

张静 冯岗 袁旭超 陈从珍 林勇   

  • 收稿日期:2009-06-09 修回日期:2009-06-30 出版日期:2009-11-05 发布日期:2009-11-05
  • 基金资助:

    中国热带农业科学院博士科研启动基金项目;中央级公益性科研院所基本科研业务费专项资助项目

Preliminary study on the Antifungal activity of thymol

  • Received:2009-06-09 Revised:2009-06-30 Online:2009-11-05 Published:2009-11-05

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摘要：

本研究采用抑制菌丝生长速率法、抑制孢子萌发法、组织测定法和盆栽试验法系统测定了百里香酚对植物病原真菌的抑制作用。结果表明，在离体条件下百里香酚对供试的10种植物病原真菌菌丝生长均有一定的抑制作用，其中，对番茄灰霉病菌的抑制作用最强，EC50为0.012 g?L-1；对番茄灰霉病菌、烟草赤星病菌和玉米大斑病菌孢子萌发有较强的抑制作用，EC50分别为0.028、0.032和0.064 g?L-1；盆栽试验结果表明百里香酚对番茄灰霉病菌具有很好的保护和治疗作用，在供试浓度为0.75 g?L-1时，对番茄灰霉病的保护和治疗作用分别为72.01%和60.28%，与对照药剂速克灵无显著性差异。

关键词: 猪血清, 猪血清, 免疫球蛋白, 提取方法, 比较

Abstract:

This study investigated the antifungal activity of alkaloids from Macleaya microcarpa in vitro and in vivo so as to reveal its antifungal activity. The bioassay results indicated that the alkaloids inhibited mycelial growth of 11 species investigated plant-pathogenic fungal in vitro. It especially could markedly inhibited the mycelial growth to Botrytis cinerea and Rhizoctomia solani. Their EC50 value against B. cinerea and R. solani were 0.022 g?L-1 and 0.024 g?L-1, respectively. The spore germination EC50 value against B. cinerea, Alternaria alternate and Exserohilum turcicum were 0.028, 0.032 and 0.064 g?L-1, respectively. In addition, protective effect and therapeutic effect of thymol (0.75 g?L-1) against the B. cinerea. were 72.01% and 60.28% in potted, respectively, which is no significant difference between Procymidone and thymol.