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中国农学通报 ›› 2009, Vol. 25 ›› Issue (22): 0-0.

• 林学 园艺 园林 •    

不知火杂柑叶片黄化对其光合特性的影响

齐笑笑 胡娟 肖家欣 周守标 申燕 杨慧   

  • 收稿日期:2009-06-16 修回日期:2009-07-07 出版日期:2009-11-20 发布日期:2009-11-20

Studies on effects of leaf chlorosis of shiranuhi on its photosynthetic characteristic

  • Received:2009-06-16 Revised:2009-07-07 Online:2009-11-20 Published:2009-11-20

摘要: 运用CI-340便携式光合测定仪对田间条件下的正常与黄化不知火杂柑的光合特性进行了测定,并通过扫描电镜观察了叶表皮特征,结果表明:(1)黄化不知火杂柑叶的叶绿素a+b含量、叶面积显著低于正常不知火(p=0.045,p=0.003),其类胡萝卜含量却显著高于正常不知火(p=0.049);二者气孔均分布于下表皮,上午8:00二者间的气孔密度与气孔开度并无明显差异。(2)两者净光合速率(Pn)日变化均呈现出“单峰”曲线,正常不知火杂柑的Pn高峰出现在8:00,较黄化不知火杂柑早2h,其中黄化不知火杂柑Pn峰值相对较低。(3)简单相关分析结果表明,对黄化不知火杂柑Pn影响显著的因子依次是气孔导度(Gs)、田间CO2浓度(Ca)、光合有效辐射(PAR)和蒸腾速率(Tr);而与正常不知火杂柑Pn呈显著相关的因子是Gs和Tr。

关键词: 加拿大一枝黄花, 加拿大一枝黄花, 水浸提液, 种子萌发

Abstract: The photosynthetic characteristics of leaves from normal and chlorotic shiranuhi under field conditions were investigated using the CI-340 portable photosynthesis system, and the leaf epidermis features of them were observed by scanning electron microscope (SEM), the main results were showed that chlorotic leaf chlorophyll a+b concentration and leaf area were significantly lower than those of normal shiranuhi (p=0.045, p=0.003), whereas carotenoid concentration was dramatically higher in chlorotic leaves than in normal leaves from shiranuhi (p=0.049), stomas were all distributed in lower epidermis of both, at a.m. 8:00, no distinct differences were detected on the stomatal frequency and apertures among them. The single peaks were found on the diurnal variations in net photosynthetic rate (Pn) of them, the peak of normal shiranuhi occurred at 8:00, in advance two hours compared with that of chlorotic shiranuhi, furthermore, the peak of Pn was relatively lower in leaves from chlorotic shiranuhi than from the normal one. Simple correlation analyses showed that the factors affecting Pn of chlorotic shranuhi could be ranked, from the highest to the lowest, as stomatal conductance(Gs)> CO2 concentration in field (Ca) >photosynthesis availability radiation(PAR)> transpiration rate(Tr), however, Pn of normal shranuhi was significantly correlated to Gs and Tr.