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中国农学通报 ›› 2009, Vol. 25 ›› Issue (23): 52-57.

所属专题: 生物技术 水稻

• 生物技术科学 • 上一篇    下一篇

水稻腈水解酶蛋白家族的生物信息学分析

王爱荣 刘向国 赵旭 鲁国东   

  • 收稿日期:2009-07-03 修回日期:2009-07-21 出版日期:2009-12-05 发布日期:2009-12-05
  • 基金资助:
    福建省自然科学基金

Bioinformatic Analysis of the Nitrilase Family in Rice

  • Received:2009-07-03 Revised:2009-07-21 Online:2009-12-05 Published:2009-12-05

摘要: 腈水解酶在植物的生命过程中起重要作用。本文对水稻中的腈水解酶进行了一系列的生物信息学分析,结果发现水稻中共有8个腈水解酶,他们都具有典型的碳氮水解酶结构域。通过ESTs搜索发现这些基因在水稻不同组织和不同胁迫条件下的的表达情况存在较大的差异。此外,对水稻的腈水解酶家族作了初步的进化分析。研究结果为进一步研究该类蛋白的分子和生物学功能奠定了良好基础。

关键词: 辐照, 辐照, 敏感性, 发芽率, 基因组

Abstract: Nitrilases are important enzyme in plants. Eight proteins were identified by searching the Oryza sativa L. ssp. japonica protein database. The bioinformatics analysis showed that they had a typical carbon-nitrogen hydrolase domain. ESTs analysis showed that each gene expressed diversely in different tissues and under different stresses, which suggested specific function of particular gene. In addition, we analyzed the evolution of the rice nitrilase family. In a summary, these data will facilitate to further characterize molecular or biological functions of the proteins.