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中国农学通报 ›› 2010, Vol. 26 ›› Issue (3): 23-26.

• 畜牧 动物医学 蚕 蜂 • 上一篇    下一篇

枯草芽孢杆菌对禽舍分离致病菌的抑菌作用研究

王城,赵杰,李旋亮,尹荣焕   

  • 收稿日期:2009-08-31 修回日期:2009-09-14 出版日期:2010-02-05 发布日期:2010-02-05
  • 基金资助:

    沈阳农业大学大学生科技创新项目

Inhibitory effect of Bacillus subtilis on pathogenic bacteria from poultry house

  • Received:2009-08-31 Revised:2009-09-14 Online:2010-02-05 Published:2010-02-05

摘要:

[目的] 研究枯草芽孢杆菌对禽舍分离致病菌的抑菌作用。[方法]采用常规方法对禽舍中采集的微生物进行分离、培养,并采用生化试验及动物试验等方法鉴定菌种及毒性,同时借助平板抑制试验检测枯草芽孢杆菌分离菌株的抑菌作用。[结果]:本研究分离的枯草芽孢杆菌菌株对致病性大肠杆菌、沙门氏菌、肠球菌属细菌均没有抑制作用,但其对变形菌属细菌、金黄色葡萄球菌却表现出不同程度的抑制作用。[结论]说明用枯草芽孢杆菌来抑制畜禽舍环境中的一些常见致病菌存在一定的潜力。

关键词: 西洋参, 西洋参, 土壤微生物, 土壤真菌, 种类, 数量

Abstract:

Bacillus subtilis, which can produce a variety of antibacterial substances, has been used as probiotics in [Objective] The present work aims to study the inhibitory effect of Bacillus subtilis on pathogenic bacteria from poultry house. [Mothed] The microorganism collected from poultry house was isolated and cultured using conventional methods, and then, the strain and toxicity was identified using biochemistry and animal experiments. At the same time, the inhibitory effect of Bacillus subtilis isolated was evaluated with restrained slab experiment. [Result] The Bacillus subtilis isolated from the present work did not inhibit the growth of pathogenic E. coli, Salmonella, and Enterococcus, but it had inhibitory effect on Proteus spp bacteria and Staphylococcus aureus with different degree. [Conclusion] Bacillus subtilis might be useful in the inhibition of common pathogenic bacteria from poultry horse.