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中国农学通报

中国农学通报 ›› 2022, Vol. 38 ›› Issue (31): 66-76.doi: 10.11924/j.issn.1000-6850.casb2021-1043

所属专题： 生物技术； 园艺

• 资源·环境·生态·土壤 • 上一篇    下一篇

间作韭菜模式下番木瓜根区微生物群落变化特征

王丽霞(), 殷晓敏, 刘永霞, 连子豪, 王必尊, 何应对()   

  1. 中国热带农业科学院海口实验站/海南省香蕉遗传改良重点实验室,海口 571101
  • 收稿日期:2021-11-03 修回日期:2022-02-23 出版日期:2022-11-05 发布日期:2022-10-27
  • 通讯作者: 何应对
  • 作者简介:王丽霞,女,1986年出生,河南洛阳人,助理研究员,硕士,研究方向：作物高效栽培。通信地址：571101 海南省海口市龙华区城西镇学院路4号 中国热带农业科学院海口实验站,Tel：0898-66774403,E-mail： wlxmm@163.com。
  • 基金资助:
    海南省自然科学基金“蕉园间作白三叶草对土壤微生态环境效应的研究”(320MS080);国家现代农业产业技术体系“国家香蕉产业技术体系海口综合试验站”(CARS-31-20)

Change Characteristics of Microbial Community in the Rhizosphere of Papaya Under Papaya-Leek Intercropping

WANG Lixia(), YIN Xiaomin, LIU Yongxia, LIAN Zihao, WANG Bizun, HE Yingdui()   

  1. Haikou Experimental Station, Chinese Academy of Tropical Agricultural Sciences/ Hainan Provincial Key Laboratory for Genetics and Breeding of Banana Genetic Improvement, Haikou 571101
  • Received:2021-11-03 Revised:2022-02-23 Online:2022-11-05 Published:2022-10-27
  • Contact: HE Yingdui

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摘要：

利用韭菜生境能释放挥发性物质,抑制根区病原真菌作用效果,研究间作韭菜模式下番木瓜根区微生物群落变化特征。在不同的间作时间提取土壤微生物DNA,应用Illumina Miseq高通量测序技术分析根区土壤微生物多样性。结果表明：（1）根区土壤细菌归属于27门64纲146目272科437属,酸杆菌门(Acidobacteria)、放线菌门(Actinobacteria)、绿弯菌门(Chloroflexi)、变形菌门(Proteobacteria)为优势菌,分别占比18.15%、22.33%、19.11%和25.53%,占全部细菌组成的85.12%;其中随着间作韭菜时间的推移,变形菌门(Proteobacteria)和酸杆菌门(Acidobacteria)的优势表现明显。（2）根区土壤真菌归属于7门24纲64目126科239属,子囊菌门(Ascomycota)、接合菌门(Zygomycota)和担子菌门(Basidiomycota)为优势菌,所占比例分别为93.71%、3.94%和1.18%,占全部细菌组成的98.83%,其中子囊菌门占主导地位。（3）在细菌属水平,间作韭菜30、60、120天,酸杆菌门的Subgroup_6_norank相对丰度最高,90天时硝化螺菌门的一个分属硝化螺菌属(Nitrospira)相对丰度最高;然而在真菌属水平,无论间作韭菜与否子囊菌门下的分属相对丰度均为最高。（4）土壤有机质、全氮和速效磷是影响番木瓜韭菜间作系统土壤微生物群落结构和土壤微生物多样性的关键因子。

关键词: 番木瓜, 番木瓜韭菜间作, 土壤养分, 土壤微生物

Abstract:

The variation characteristics of microbial community in rhizosphere of papaya under papaya-leek intercropping pattern were studied by using volatile substances released in leek habitat to inhibit the pathogenic fungi in the rhizosphere of papaya. Soil microbial DNA was extracted at different intercropping periods, and soil microbial diversity in rhizosphere was analyzed by Illumina Miseq high-throughput sequencing technology. The results showed that: (1) the soil bacteria in the rhizosphere were classified into 27 phyla, 64 classes, 146 orders, 272 families and 437 genera, Acidobacteria, Actinobacteria, Chloroflexi and Proteobacteria were the dominant bacteria, and their proportions were 18.15%, 22.33%, 19.11%, and 25.53%, respectively, accounting for 85.12% of the total bacterial composition; with the increase of intercropping time, the advantages of Proteobacteria and Acidobacteria were obvious; (2) the soil fungi of rhizosphere were classified into 7 phyla, 24 classes, 64 orders, 126 families and 239 genera, Ascomycota, Zygomycota and Basidiomycota were the dominant, and their proportions were 93.71%, 3.94% and 1.18%, respectively, accounting for 98.83% of the total composition; and Ascomycota was the dominant; (3) at the genus level of bacteria, the relative abundance of Subgroup _6_ norank of Acidobacteria was the highest at 30, 60 and 120 d of intercropping; at 90 d, the relative abundance of Nitrospira was the highest; at the genus level of fungi, the relative abundance of the genus of Ascomycota was the highest whether papaya was intercropped with leek or not; (4) soil organic matter, soil total nitrogen and soil available phosphorus in the rhizosphere were the key factors affecting the soil microbial community structure and soil microbial diversity in papaya-leek intercropping pattern.

Key words: papaya, papaya-leek intercropping, soil nutrients, soil microorganism

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