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中国农学通报

中国农学通报 ›› 2010, Vol. 26 ›› Issue (3): 64-67.

所属专题： 生物技术； 园艺

• 生物技术科学 • 上一篇    下一篇

利用柱头涂抹法进行西瓜T-DNA导入和分子标记研究

包文风,马双武,王吉明,尚建立,王小军   

  • 收稿日期:2009-09-01 修回日期:2009-11-06 出版日期:2010-02-05 发布日期:2010-02-05
  • 基金资助:

    农业部作物种质资源保护项目;国家科技基础条件平台项目

Research of the T-DNA Introduced to Watermelon and Molecular Marker Via the Stigma Smeared Method

  • Received:2009-09-01 Revised:2009-11-06 Online:2010-02-05 Published:2010-02-05

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摘要：

摘要：借鉴拟南芥蘸花进行外源DNA导入的方法，通过柱头涂抹进行西瓜T-DNA导入和分子标记研究。结果表明：品种ZXG01078坐果率最高，且有变异苗产生，是较为理想的介导品种；卡那霉素筛选处理西瓜种子的最佳浓度500～700mg/L，不同品种间有差异；卡那霉素筛选处理西瓜叶片的最佳部位为嫩叶或幼叶，最佳浓度4000～8000mg/L，不同品种间差异不明显。处理后代中无心苗和连体苗的稳定产生，说明外源基因已进入并干扰了其正常的生长发育过程。

关键词: 烤烟, 烤烟, 叶表面, 蔗糖酯, 化学成分, 相关性

Abstract:

Abstract：From the method of the foreign DNA introduced to Arabidopsis thaliana via flowers dip, we used stigma smear method to transfer T-DNA into watermelon and research T-DNA molecular marker. The results showed the ideal tranform specie is ZXG01078 with the highest fruit setting rate and the most deviant seedlings. The best concentration of the watermelon seeds treated by kanamycin is 500～700mg/L with differences among the species; the best concentration of the watermelon leaf treated by kanamycin is 4000～8000mg/L, and the best position is spire leaf or youngleaf with no difference among the species. The steadily variation appearing of no pointing and twinborn seedling indicated the normal growth interfered by foreign DNA in the progeny.