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中国农学通报

中国农学通报 ›› 2010, Vol. 26 ›› Issue (3): 59-63.

所属专题： 生物技术

• 生物技术科学 • 上一篇    下一篇

EST分子标记在基因组学中应用的研究进展

陈全求,詹先进,蓝家样,黄云,符家平   

  • 收稿日期:2009-09-08 修回日期:2009-11-01 出版日期:2010-02-05 发布日期:2010-02-05

Study progress on Application of EST (expressed sequence tags) in the functional genomics

  • Received:2009-09-08 Revised:2009-11-01 Online:2010-02-05 Published:2010-02-05

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摘要：

植物EST计划作为植物基因组计划的一个重要组成部分，本文概括介绍了几种主要EST标记(EST-SSR, EST-PCR, EST-SNP, EST-AFLP, EST-RFLP)在比较基因组学，系统发育学，基因功能研究等方面的应用情况，并对EST标记的应用前景作了展望。

关键词: 植物多糖, 植物多糖, 结构, 分离纯化

Abstract:

The plant EST (expressed sequence tags) program is one of the most important parts which compose the plant genome program. The expansion of EST data base is foundation of development of EST markers. In this paper, the development of five EST markers (EST-SSR, EST-PCR, EST-SNP, EST-AFLP, and EST-RFLP) and their application on comparative genomics, phylogeny and function studies of gene. Some problem exit in the development of EST markers is also discussed.