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中国农学通报 ›› 2010, Vol. 26 ›› Issue (8): 104-107.

• 农学 农业基础科学 • 上一篇    下一篇

2个朝天椒变种农艺性状调查及指纹图谱构建研究

蒋向辉1,2,3,佘朝文1,2,3,许栋1,张青桦1   

  • 收稿日期:2009-10-14 修回日期:2009-11-29 出版日期:2010-04-20 发布日期:2010-04-20

Study on agronomic traits investigation and fingerprint construction of two varieties of capsicum frutescen Var

Jiang Xianghui1,2,3, She Chaowen1,2,3, Xu Dong1, Zhang Qinghua1   

  • Received:2009-10-14 Revised:2009-11-29 Online:2010-04-20 Published:2010-04-20

摘要:

本文从株展最宽、单果长、叶长、心室数、果肉厚、种子数等农艺性状对两个朝天椒品种进行了比较分析,结果显示所考察的14 个性状,除株展最窄与心室数两性状在两品种间没有显著性差异外,其他性状在两品种间的差异都达到极显著水平。通过RAPD引物的筛选及RAPD反应体系的优化,分别找到了12个与两品种特有农艺状状紧密连锁的分子标记,构建了地方朝天椒品种的特有指纹图谱,有利用于从分子水平对两品种进行准确区分,为特有地方辣椒品种的保护提供了参考。

关键词: 洋葱伯克氏菌群, 洋葱伯克氏菌群, 基因型, 多样性

Abstract:

This study made a comparative analysis between two kinds of capsicum var, with similar agronomic trait -s,from their plant width,length of fruit and leaves,amounts of ventricular myocytes,pulp thickage,number of seeds etc.It was found that 14 properties researched between the two pepper species have significant difference except pla -nt width and ventricular myocytes of two properties,which have no notable difference,.12 molecular marks were sel -ected which have the specificity banding during two varieties by screening of RAPD primer and optimization of RAPD system. Special fingerprint of local capsicum var was constructed,which provided information for the local pepper species pretection.