欢迎访问《中国农学通报》，
中国农学通报

中国农学通报 ›› 2010, Vol. 26 ›› Issue (8): 234-236.

所属专题： 农业地理

• 植物保护 农药 • 上一篇    下一篇

桔小实蝇5个地理种群的SSR遗传多态性分析

姚廷山,胡军华,李鸿筠,冉春,刘浩强,雷慧德,肖田   

  • 收稿日期:2009-10-14 修回日期:2009-11-04 出版日期:2010-04-20 发布日期:2010-04-20
  • 基金资助:

    农业部科研院所社会公益研究专项

Microsatellite markers for genetic polymorphism in five geographic population of the Bactrocera dorsalis (Hendel)

Yao Tingshan, Hu Junhua, Li Hongjun, Ran Chun, Liu Haoqiang, Lei Huide, Xiao Tia   

  • Received:2009-10-14 Revised:2009-11-04 Online:2010-04-20 Published:2010-04-20

PDF (PC)

983

摘要：

摘要：桔小实蝇Bactrocera (Bactrocera) dorsalis (Hendel)是一种为害多种水果、瓜类、茄类蔬菜的检疫性害虫，食性杂、寄主范围广，直接威胁水果和蔬菜的安全生产。笔者运用改进的CTAB法提取单头实蝇的基因组DNA，形态鉴定及特异性引物BD1/BD2 PCR鉴定桔小实蝇，用7对微卫星标记（SSR）引物对采自我国福建、海南、广东、云南和四川5省的桔小实蝇种群间的遗传关系进行初步分析，DPS聚类分析结果显示，福建桔小实蝇与其他几个地区的桔小实蝇的差异性较大，达到0.86，另外4省之间遗传距离较近。

关键词: 苹果, 苹果, 花序, 几何造型, 三维可视化

Abstract:

Abstract: The fruits flies Bactrocera (Bactrocera) dorsalis (Hendel) is one of the major economically important and quarantine insects in Asia. Genomic DNA of the sampled populations came from Fujian, Hainan, Guangdong, Yunnan and Sichuan，was extracted from thoracic tissue of individual adult ?ies by CTAB method and amplified with the polymerase chain reaction. By using microsatellite markers (SSR), the fingerprint of five geographic population was investigated. With good reproducibility and high polymorphic, seven microsatellite primers were utilized in this study to assess the genetic polymorphism of orient fruit fly primarily. The results indicated that genetic differentiation exists among the five populations.