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中国农学通报

中国农学通报 ›› 2010, Vol. 26 ›› Issue (8): 33-37.

所属专题： 生物技术

• 畜牧 动物医学 蚕 蜂 • 上一篇    下一篇

口蹄疫病毒(OS/99株)VP1蛋白的空间构型和其可能抗原位点的初步分析

刘靖清1,王君1,周兵1,李凯2   

  • 收稿日期:2009-11-17 修回日期:2009-12-05 出版日期:2010-04-20 发布日期:2010-04-20

Research of the spatial arrange and its possible antigenic sites in vp1 protein of FMDV from OS/99 strain

Liu Jingqing1, Wang Jun1, Zhou Bing1, Li Kai2   

  • Received:2009-11-17 Revised:2009-12-05 Online:2010-04-20 Published:2010-04-20

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摘要：

本试验研究了经BHK-21培养的口蹄疫病毒（FMDV）OS/99株VP1蛋白的分子免疫学特征。通过RT-PCR得到FMDV的VP1基因序列，应用分子生物学软件对其蛋白序列的空间构型和抗原性进行分析。通过对其β-折叠数量、亲水性、抗原指数、可塑性、表面可能性的抗原性参数的综合分析，得出OS/99株VP1蛋白中可能含有多个抗原位点，具有较强的抗原覆盖性。抗原位点可能位于22-29、43-46、82-85、92-93、100-111、134-139、154-156、180-184和196-203位氨基酸残基区域内。毒株经过BHK-21细胞培养13代后，其蛋白空间构型未发生明显变化。

关键词: 春谷子, 春谷子, 生育期, 氮、磷、钾, 吸收特点

Abstract:

The aim of our experiment was to investigate molecular immunological characters of VP1 in foot-and-mouth disease virus from OS/99 viral strain cultured in BHK-21 cells . In this study,the sequence of VP1 ,which was isolated through RT-PCR , was analyzed in DNAstar software to abtain its spatial arrange and antigenicity . The result of this research indicated that the VP1 from OS/99 might contain many antigenic sites in terms of some parameters on the antigenicity , which located in 22-29，43-46，82-85，92-93，100-111，134-139，154-156，180-184，196-203 amino acid regions . For that , the virus strain of os/99 showed the powerful ability in aspect of more broader coverage in antigenicity Together ,it was suggested that the spatial conformation of VP1 in OS/99 virus strain ,which were subcultured for 13 generations , have not been obviously changed .