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中国农学通报 ›› 2010, Vol. 26 ›› Issue (5): 209-212.

• 林学 园艺 园林 • 上一篇    下一篇

春荷鼎杂交根状茎的组培快繁的研究

潘银萍1,李承秀2,王长宪2,张东旭1,王丰妍1   

  • 收稿日期:2009-12-11 修回日期:2009-12-25 出版日期:2010-03-05 发布日期:2010-03-05

Study of the rootstocks about Cymbidium faberi ’ chun he ding’ in Rapid Multiplication Techniques

  • Received:2009-12-11 Revised:2009-12-25 Online:2010-03-05 Published:2010-03-05

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摘要：

摘要：【研究目的】本文采用传统品种春荷鼎与普通春兰杂交的继代根状茎为试材，对根状茎的增殖和分化进行研究。【方法】探讨不同培养基、转换培养基、6—BA与NAA、IBA对根状茎增殖、分化的影响。【结果】不同培养基对生长率有明显影响，1/2 MS为春兰根状茎的基本培养基；最适增殖培养基为1/2 MS+BA0.1+NAA0.1；根状茎的最佳分化培养基为1/2 MS+BA1.0+IBA1.0；以1/2 MS+BA1.0+IBA1.0培养基再转入B5+BA1.0+IBA1.0培养得交替培养方式，获得分化率达76%。【结论】组培快繁是培育春兰新品种的重要技术手段。

关键词: 香草兰, 香草兰, 化学成分, 药用功效, 保健作用

Abstract:

Abstract: 【OBJECTIVE】Taking the rootstocks of Cymbidium goeringii as test pieces, Cymbidium faberi ’ chun he ding’ and common Cymbidium faberi were employ as parents, study on Multiplication and differentiation of rhizomes in vitro of Cymbidium goeringii. 【METHOD】 Four factors of medium ,6-BA and NAA、IBA were design to study the effects on differentiation of rhizome. 【RESULTS】 The result show that the growth rate was affected evidently by media,The 1/2 MS was used as basal medium,The optimal Multiplication Conditions For therootstocks were that 1/2MS +BA0.1 +NAA0.1, The best differentiation medium was1/2 MS+BA1.0+IBA1.0,Firstly using 1/2MS + BA1.0 + IBA1.0 and then using B5 + BA1.0 +IBA1.0 could gain a higher differentiation rate of the rootstocks to 76 Percentage. 【CONCLUSION】Rapid Multiplication Techniques were Cultivation Cymbidium faberi variety import technological means .