关键词: 中介蝮蛇, 中介蝮蛇, 纤溶酶, 基因克隆, 序列分析

Abstract:

In this paper, the allelopathy of the extracts of petroleum ether, methanol, ethyl acetate, aether and water from seed coat and endosperm of Taxus chinensis var．mairei was studied through bioassay method. The results showed that there are inhibitory effects in extracts on the germination and growth of cabbage, and the inhibitory effects become stronger as the contents of extraction solutions increase. the extract of methanol from seed coat and endosperm on germination and growth inhibitory is the strongest, and the inhibition rate is 100%, The inhibitory effect on the extracts of petroleum ether, aether and ethyl acetate is stronger than the endosperm in seed coat, the inhibition of extracts of water of endosperm is stronger than the seed coat. To inhibit the growth of cabbage seedling height, the inhibition of extracts of water of seed coat is stronger than the endosperm in low concentrations, high-density processing is the opposite. To inhibit the growth of cabbage root length, the inhibition of extracts of petroleum ether and ethyl acetate of seed coat is stronger than the endosperm, and the inhibition of extracts of aether and water is the opposite. The inhibitory effects of endosperm extractions to the growth of cabbage seedling height can be taxised as, in turn from strong to weak, the extraction phase of methanol, ethyl acetate, aether, petroleum ether and water; those to the growth of cabbage seedling root and germination percentage can be taxised as, methanol, aether, ethyl acetate, water and petroleum ether. The inhibitory effects of seed coat extraction solutions to the growth of cabbage seedling height and root can be taxised as, of methanol, of petroleum ether, of ethyl acetate, of aether and of water. those to the growth of cabbage seedling germination percentage can be taxised as, methanol, aether, ethyl acetate, petroleum ether and water.