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中国农学通报

中国农学通报 ›› 2010, Vol. 26 ›› Issue (9): 277-282.

所属专题： 园艺

• 植物保护 农药 • 上一篇    下一篇

番茄内生细菌种群动态分析及拮抗菌株的筛选

王美琴1,刘慧平1,韩巨才1,路涛2   

  • 收稿日期:2009-12-25 修回日期:2010-01-12 出版日期:2010-05-05 发布日期:2010-05-05
  • 基金资助:

    山西省自然基金项目;山西省回国留学人员科研资助项目;山西省回国留学人员科研资助项目;山西省科技攻关项目

Population Dynamics of Endophytic Bacteria in Tomato Plant and Screening of Antagonistic Strains

Wang Meiqin1, Liu Huiping1, Han Jucai1, Lu Tao2   

  • Received:2009-12-25 Revised:2010-01-12 Online:2010-05-05 Published:2010-05-05

PDF (PC)

998

摘要：

摘要：采用研磨组织稀释分离法对3个种植地区的番茄内生细菌的种群分布、数量变化规律进行了初步探讨。共分离、纯化得到内生细菌374株，经形态及理化特征鉴定,分属10个不同的属，13个种。其中假单孢菌属Pseudomonas sp.，黄单孢菌属Xanthomonas sp.；肠杆菌属Entenobacter sp.和芽孢杆菌属Bacillus sp.为优势种群。内生细菌在番茄植株种群数量从0.21x104 到13.82x104 cfu.g -1鲜重之间，但不同品种、组织、生育期及种植地，内生细菌的种群和数量各不相同，各组织中内生细菌的种群及数量根中最多，其次是茎和叶，成株期内生细菌无论种类和数量都明显高于苗期。采用平板对峙法从分离的内生细菌中筛选出对番茄灰霉病菌具有体外拮抗活性的菌株32个，占菌株总数的8.56%； 25个菌株对叶霉病菌有抑制作用，占菌株总数的6.68%；对灰霉病菌和叶霉病菌都具有拮抗作用的菌株20个，占菌株总数的5.35%。对灰霉病菌有强拮抗作用的菌株12个，对叶霉病菌有强拮抗作用的菌株仅1个，其中芽孢杆菌是主要的类群。

关键词: 芽孢杆菌, 芽孢杆菌, 抗菌物质, 发酵

Abstract:

Abstract：In order to study distribution and population dynamics of endophytic bacteria in tomato plant, 374 endophytic bacteria strains of tomato plants were isolated and purified from 3 cultivated regions. By conventional methods, these strains were identified as 13 genus. Among them, Bacillus,Pseudomonas,Xanthomonas and Entenobact were the dominant genus.The endophytic bacteria were the most common in the tomato plants. Results indicated that number of endophytic bacteria ranged from 0.21x104 to 13.82x104 cfu.g -1fresh weight，but there were distinguish between the number and variety of endophytic bacteria in different tissue，different variety, different ages and different cultivated regions. The number of endophytic bacteria was the largest in roots, followed by in stems and leaves，and the number and variety of endophytic bacteria in Maturity plants were higher than in seedling plants. Of all isolates，32 strains were known to express antibiosis activities against Botrity cineane , and 25 strains were known to express antibiosis activities against Fulvia fulva，20 strains were determined in vitro antagonism to both of the pathogens，there have been 12 strong antagonistic strains against Botrity cineane,but only 1 against Fulvia fulva.. Bacillus sp.were the dominant genus.